{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T05:41:25Z","timestamp":1696570885608},"reference-count":2,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,22]],"date-time":"2014-03-22T00:00:00Z","timestamp":1395446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Our recent publication \u201cInability of a monoclonal anti\u2010light chain antibody to detect clonal plasma cells in a patient with multiple myeloma by multicolor flow cytometry,\u201d underlined the importance of choice of antibodies to detect cytoplasmic light chains. Our present study extends this finding for detection of surface immunoglobulin (<jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>g) light chains on clonal <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Multicolor flow cytometry was used for analyzing bone marrow (<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>) from a patient with a <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>10\u2010positive follicular lymphoma for infiltrating clonal <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content> aspirate, <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells could be identified expressing <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>19, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>10, and high levels of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>20. No <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>g light chain expression was found on this population of <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells employing monoclonal antibodies. Re\u2010analysis using polyclonal antibodies against <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>g light chains, revealed presence of lambda light chains on the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>10positive <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data illustrate when antibodies against <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>g light chains are employed for <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell clonality assessment, polyclonal antibodies are preferred over monoclonal antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21716","type":"journal-article","created":{"date-parts":[[2014,3,22]],"date-time":"2014-03-22T18:57:10Z","timestamp":1395514630000},"page":"493-495","source":"Crossref","is-referenced-by-count":1,"title":["Inability of Monoclonal Anti\u2010light Chain Antibody to Detect Clonal B\u2010Cells in a Patient with Follicular Lymphoma by Multicolor Flow Cytometry"],"prefix":"10.1002","volume":"28","author":[{"given":"Jeroen F.","family":"van Velzen","sequence":"first","affiliation":[{"name":"Section of Medical Immunology, Laboratory of Translational Immunology University Medical Centre Utrecht  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorine","family":"van den Blink","sequence":"additional","affiliation":[{"name":"Section of Medical Immunology, Laboratory of Translational Immunology University Medical Centre Utrecht  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid E.H.","family":"Wiegers","sequence":"additional","affiliation":[{"name":"Section of Medical Immunology, Laboratory of Translational Immunology University Medical Centre Utrecht  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andries C.","family":"Bloem","sequence":"additional","affiliation":[{"name":"Section of Medical Immunology, Laboratory of Translational Immunology University Medical Centre Utrecht  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,22]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21044"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.05.020"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21716","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T16:42:28Z","timestamp":1696524148000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,22]]},"references-count":2,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1002\/jcla.21716"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21716","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,22]]}}}