{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:57:09Z","timestamp":1790193429165,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,28]],"date-time":"2014-03-28T00:00:00Z","timestamp":1395964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Unit\u00e9 de recherche, Auto-immunit\u00e9 et Allergie","award":["03\/UR\/07-02"],"award-info":[{"award-number":["03\/UR\/07-02"]}]},{"name":"Facult\u00e9 de Pharmacie de Monastir, Universit\u00e9 de Monastir, Tunisia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL IgG, IgA, and IgM) and beta 2 glycoprotein I (a\u03b22GPI IgG, IgA, and IgM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of antiphospholipid antibodies (aCL and\/or a\u03b22GPI) was significantly higher in PBC patients than in controls (70 vs. 5%, <jats:italic>P<\/jats:italic> &lt; 10<jats:sup>\u22126<\/jats:sup>). The frequency of aCL antibodies (IgG, IgA or IgM) was significantly higher in PBC patients than in the control group (23.7 vs. 3.7%, <jats:italic>P<\/jats:italic> = 0.0005). The frequencies of aCL IgA and aCL IgM in PBC patients' sera were significantly higher than those in the control group (10 vs. 0%, <jats:italic>P<\/jats:italic> = 0.003 and 20 vs. 2.5%, <jats:italic>P<\/jats:italic> = 0.001, respectively). Two patients of eighty (2.5%) had aCL IgG, aCL IgA and aCL IgM. The frequency of a\u03b22GPI antibodies (IgG, IgA, or IgM) was significantly higher in PBC patients than in the control group (70 vs. 1.2%, <jats:italic>P<\/jats:italic> &lt; 10<jats:sup>\u22126<\/jats:sup>). The frequencies of a\u03b22GPI IgG, a\u03b22GPI IgA, and a\u03b22GPI IgM in PBC patients' sera were significantly higher in patients than in the control group (12.5 vs. 0%, <jats:italic>P<\/jats:italic> = 0.003; 62.5 vs. 1.2%, <jats:italic>P<\/jats:italic> &lt; 10<jats:sup>\u22126<\/jats:sup>; and 21.2 vs. 0%, <jats:italic>P<\/jats:italic> &lt; 10<jats:sup>\u22124<\/jats:sup>, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Autoantibodies related to APLS (aCL and a\u03b22GPI) were present in the majority of patients with PBC, reflecting the ability of these antibodies to engage mediators of damage.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21723","type":"journal-article","created":{"date-parts":[[2014,3,29]],"date-time":"2014-03-29T00:36:59Z","timestamp":1396053419000},"page":"32-36","source":"Crossref","is-referenced-by-count":20,"title":["High Frequency of Antiphospholipid Antibodies in Primary Biliary 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