{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T05:10:30Z","timestamp":1696482630389},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,5,5]],"date-time":"2014-05-05T00:00:00Z","timestamp":1399248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MEXT\/JSPS KAKENHI","award":["23590677","24590697"],"award-info":[{"award-number":["23590677","24590697"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>We evaluated the DiversiLab (DL) system with universal primers, a semiautomated repetitive extragenic palindromic sequence\u2010based polymerase chain reaction (PCR) (rep\u2010PCR) system, for the characterization of <jats:italic>Helicobacter pylori<\/jats:italic> in Japan. All 135 isolates from Japanese patients with gastric cancer (GC, <jats:italic>n<\/jats:italic> = 55) or non\u2010GC (<jats:italic>n<\/jats:italic> = 80) were used and subjected to the drug susceptibility examinations (amoxicillin, AMPC; metronidazole, MNZ; and clarithromycin, CAM) by E\u2010test. There were 28 MNZ\u2010resistant (20.7%), 35 CAM\u2010resistant (25.9%), and 16 MNZ\/CAM\u2010resistant (11.9%) isolates. DL rep\u2010PCR fingerprinting analysis at the level of 95% similarity revealed five major groups (A\u2013E) and the other including 45 isolates. The occupation rates of GC\u2010derived isolates in groups B (54.2%) and E (58.8%) were higher than in the other groups: A (26.7%), C (28.6%), D (30.0%), and the other (40.0%). Relative higher occupation rates of drug resistants, such as MNZ\u2010, CAM\u2010 and double MNZ\/CAM\u2010resistant isolates, were observed in groups B (45.8%), C (42.6%), and D (40%). Five of eight GC\u2010derived isolates with MNZ\/CAM resistance were significantly assigned to group B (<jats:italic>P<\/jats:italic> = 0.0312, \u03c7<jats:sup>2<\/jats:sup>\u2010test). These results suggest that the isolates classified in group B have a potential to contribute to the development of severe gastric disorders. The DL system, rapid and high sensitive technology, would be widely available in clinical laboratory for pathological and epidemiological analyses even in <jats:italic>H. pylori<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/jcla.21758","type":"journal-article","created":{"date-parts":[[2014,5,6]],"date-time":"2014-05-06T01:36:55Z","timestamp":1399340215000},"page":"250-253","source":"Crossref","is-referenced-by-count":4,"title":["Clinical Application of the DiversiLab Microbial Typing System Using Repetitive Sequence\u2010Based PCR for Characterization of <i>Helicobacter pylori<\/i> in Japan"],"prefix":"10.1002","volume":"29","author":[{"given":"Norihito","family":"Morimoto","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Medicine Kochi University School of Medicine  Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshie","family":"Nishida","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"},{"name":"Clinical Laboratory Medicine Kochi University School of Medicine  Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mie","family":"Morisawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoe","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamae","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyuki","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chizuko","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Medicine Kochi University School of Medicine  Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Matsumura","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Medicine Kochi University School of Medicine  Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kochi Medical School Hospital, Kochi University  Kohasu, Oko\u2010cho, Nankoku Kochi Japan"},{"name":"Clinical Laboratory Medicine Kochi University School of Medicine  Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-2-665"},{"issue":"12","key":"e_1_2_2_3_1","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1128\/JCM.37.12.4071-4080.1999","article-title":"Performance criteria of DNA fingerprinting methods for typing of Helicobacter pylori isolates: Experimental results and meta\u2010analysis","volume":"37","author":"Burucoa C","year":"1999","journal-title":"J Clin 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