{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T10:32:31Z","timestamp":1786789951755,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T00:00:00Z","timestamp":1411084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Autoimmune hepatitis (AIH) is a rare condition characterized by the presence of autoantibodies distinctive of type 1 AIH (AIH\u20101) and type 2 AIH (AIH\u20102). The aim of this study was to evaluate the autoantibody profile in a cohort of pediatric and adult AIH patients, using both indirect immunofluorescence (IIF) and a new multiplexed line\u2010blot assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera from 63 pediatric and 53 adult AIH patients were tested for antinuclear (ANA), antismooth muscle (SMA), anti\u2010liver kidney microsome 1 (anti\u2010LKM1), anti\u2010liver cytosol 1 (anti\u2010LC1) autoantibodies using IIF methods; for anti\u2010LKM1, anti\u2010LC1, and soluble liver antigen\/liver\u2010pancreas (anti\u2010SLA\/LP) autoantibodies using the line\u2010blot; for anti\u2010F\u2010actin autoantibodies using IIF both on VSM47 cell\u2010line and on rat intestinal epithelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AIH\u20101 was the most common type of AIH in the adult cohort (73.6%), while AIH\u20102 was the most common AIH in the pediatric cohort (61.9%). Both in adult and pediatric AIH\u20102 anti\u2010LKM1 were the prevalent autoantibodies. In pediatric AIH\u20102 anti\u2010LC1 autoantibodies were more frequent than in adult AIH\u20102 (59 vs. 28.6%), and in 35.9% of cases they were present alone. In 17 patients anti\u2010LC1 autoantibodies were detected only with the line\u2010blot assay. The levels of anti\u2010LKM1 and of anti\u2010LC1 were not different between adult and pediatric AIH, and the overall agreement between the results obtained with the two IIF methods for F\u2010actin detection was 98.8% (CI 95%: 94.4\u201399.7%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The line\u2010blot assay showed a higher sensitivity than IIF for anti\u2010LC1 detection. Anti\u2010LKM1 and anti\u2010LC1 autoantibody levels are not different in adults and children. An almost perfect agreement between the two IIF methods for anti\u2010F\u2010actin detection has been observed.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21813","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T16:23:34Z","timestamp":1411143814000},"page":"41-46","source":"Crossref","is-referenced-by-count":34,"title":["Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis"],"prefix":"10.1002","volume":"30","author":[{"given":"Danilo","family":"Villalta","sequence":"first","affiliation":[{"name":"Allergologia e Immunologia Clinica A.O. \u201cS. Maria degli Angeli\u201d  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elia","family":"Girolami","sequence":"additional","affiliation":[{"name":"Dipartimento di Oncoematologia Pediatrica e Medicina Trasfusionale Ospedale Bambino Ges\u00f9  Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Alessio","sequence":"additional","affiliation":[{"name":"Laboratorio Analisi Chimico\u2010Cliniche Ospedali Riuniti di Bergamo  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Concetta","family":"Sorrentino","sequence":"additional","affiliation":[{"name":"Laboratorio Analisi Chimico\u2010Cliniche e Microbiologiche ISMETT  Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilina","family":"Tampoia","sequence":"additional","affiliation":[{"name":"Laboratorio di Patologia Clinica Policlinico Consorziale di Bari  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignazio","family":"Brusca","sequence":"additional","affiliation":[{"name":"Patologia Clinica Ospedale Buccheri La Ferla  Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Daves","sequence":"additional","affiliation":[{"name":"Laboratorio Centrale Ospedale Civile  Merano (BZ) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brunetta","family":"Porcelli","sequence":"additional","affiliation":[{"name":"Dipartimento Biotecnologie Mediche Universit\u00e0 di Siena  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Barberio","sequence":"additional","affiliation":[{"name":"Patologia Clinica Ospedale C\u00e0 Foncello  Treviso Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariaelisabetta","family":"Conte","sequence":"additional","affiliation":[{"name":"Allergologia e Immunologia Clinica A.O. \u201cS. Maria degli Angeli\u201d  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Pantarotto","sequence":"additional","affiliation":[{"name":"Allergologia e Immunologia Clinica A.O. \u201cS. Maria degli Angeli\u201d  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Bizzaro","sequence":"additional","affiliation":[{"name":"Patologia Clinica Ospedale Civile  Tolmezzo (UD) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"on behalf of the Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840180435"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80297-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22322"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92024-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840180102"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.05479.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/0891693021000056712"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930701619227"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(00)80425-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.3374"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.027367"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930701619896"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/00313025.2010.493867"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2013.08.004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1233529"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2013.11.009"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.08.002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2011.02603.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510250308"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinre.2011.10.013"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21813","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T17:44:11Z","timestamp":1696355051000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,19]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1002\/jcla.21813"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21813","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,19]]}}}