{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:43:57Z","timestamp":1790358237553,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,10]],"date-time":"2014-11-10T00:00:00Z","timestamp":1415577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Celiac disease (CD) is an immune\u2010mediated intolerance to dietary gluten, affecting genetically predisposed individuals. ELISA\u2010based serological tests help to decide if further duodenal biopsy is necessary, for this the diagnostic kits have to be accurate, specific, and sensible. In this study, we investigate the performance of an ELISA that uses the purified cross\u2010linked complex of tissue transglutaminase and gliadin, referred as the \u201cneoepitope\u201d (<jats:italic>AESKULISA<\/jats:italic>\u00ae tTG New Generation), as antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated 41 newly diagnosed celiac patients, 18 celiac patients on gluten\u2010free diet, and 169 controls, comprising healthy subjects, patients affected by other autoimmune diseases, and patients affected by several non\u2010autoimmune diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Conclusion<\/jats:title><jats:p>The assay has an excellent performance. Due to its high level of diagnostic accuracy, this assay constitutes a new approach for the screening of celiac patients not only for the diagnosis of CD, but also for monitoring patients on gluten\u2010free diet and their compliance. Moreover, cases of neoepitope\u2010positive subjects who were tested negative with \u201cclassical\u201d serological markers could have a predictive value for this pathology. This aspect will require further studies of elaboration.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21816","type":"journal-article","created":{"date-parts":[[2014,11,10]],"date-time":"2014-11-10T20:08:46Z","timestamp":1415650126000},"page":"65-70","source":"Crossref","is-referenced-by-count":15,"title":["Assessment of a Test for the Screening and Diagnosis of Celiac Disease"],"prefix":"10.1002","volume":"30","author":[{"given":"Brunetta","family":"Porcelli","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnologies Biochemistry Division University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Ferretti","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Neuroscience University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Vindigni","sequence":"additional","affiliation":[{"name":"Oncological Department, Pathological Anatomy Division Siena University Hospital  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Terzuoli","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies Biochemistry Division University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.22123"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074129"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204521200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310198200"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2004.06.009"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2003.02326.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12989-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000073710"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00239-5"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.56.5.389"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.11.1567"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.13.1.150-151.2006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2006.05.004"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-007-0058-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.845"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e3181c992be"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2009.07.011"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.02.060"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.128132"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-009-8160-z"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199910000-00019"},{"key":"e_1_2_6_23_1","unstructured":"NIH Consensus Development Conference on Celiac Disease Bethesda2004. 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