{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:28:37Z","timestamp":1740104917923,"version":"3.37.3"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T00:00:00Z","timestamp":1419552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>Cryptorchidism represents a risk factor for infertility and germ cell testicular neoplasia. An increased rate of cryptorchidism has been reported in subjects with Down's syndrome. Cyclic nucleotide phosphodiesterases (PDEs) are important messengers that regulate and mediate a number of cellular responses to extracellular signals, such as neurotransmitters and hormones. PDE4B, cAMP\u2010specific (PDE4B) gene which maps to chromosome 1p31.3 appears to be involved in schizophrenia, chronic psychiatric illness, learning, memory, and mood disturbances. Expression of PDE4 enzymes have been studied in testes of cryptorchid rats. Expression of PDE4B protein examination showed marked degenerative changes in the epithelial lining of the seminiferous tubules. These findings led us to evaluate PDE4 mRNA expression in leukocytes of peripheral blood of five men with DS and cryptorchidism and eleven subjects with DS without cryptorchidism compared with healthy men (controls) by quantitative Real Time PCR (qRT\u2010PCR).<\/jats:p><jats:p>This study showed that the <jats:italic>PDE4B<\/jats:italic> gene was downexpressed in men with DS and cryptorchidism compared to normal controls and DS without cryptorchidism. A lower expression of the <jats:italic>PDE4B<\/jats:italic> gene may be involved in the neurological abnormalities in subjects with Down's syndrome. Moreover, <jats:italic>PDE4B<\/jats:italic> gene may be involved in the testicular abnormalities of men with DS and cryptorchidism.<\/jats:p>","DOI":"10.1002\/jcla.21835","type":"journal-article","created":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T23:37:12Z","timestamp":1419637032000},"page":"196-199","source":"Crossref","is-referenced-by-count":3,"title":["Expression of Phosphodiesterase 4B cAMP\u2010Specific Gene in Subjects With Cryptorchidism and Down's Syndrome"],"prefix":"10.1002","volume":"30","author":[{"given":"Michele","family":"Salemi","sequence":"first","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosita A.","family":"Condorelli","sequence":"additional","affiliation":[{"name":"Section of Endocrinology Andrology and Internal Medicine Department of Medical and Pediatric Sciences University of Catania  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro","family":"La Vignera","sequence":"additional","affiliation":[{"name":"Section of Endocrinology Andrology and Internal Medicine Department of Medical and Pediatric Sciences University of Catania  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Castiglione","sequence":"additional","affiliation":[{"name":"Section of Endocrinology Andrology and Internal Medicine Department of Medical and Pediatric Sciences University of Catania  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Salluzzo","sequence":"additional","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmela M.","family":"Bonaccorso","sequence":"additional","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirella","family":"Vinci","sequence":"additional","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Bosco","sequence":"additional","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmelo","family":"Romano","sequence":"additional","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Campagna","sequence":"additional","affiliation":[{"name":"Section of Endocrinology Andrology and Internal Medicine Department of Medical and Pediatric Sciences University of Catania  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1049-0683","authenticated-orcid":false,"given":"Corrado","family":"Romano","sequence":"additional","affiliation":[{"name":"IRCCS Oasi Institute for Research on Mental Retardation and Brain Aging  Troina EN Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo E.","family":"Calogero","sequence":"additional","affiliation":[{"name":"Section of Endocrinology Andrology and Internal Medicine Department of Medical and Pediatric Sciences University of Catania  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/S0039-6109(16)39190-3","article-title":"Current concepts in the management of the undescended testis","volume":"50","author":"Fonkalsrud EW","year":"1970","journal-title":"Surg Clin North Am"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-010-4072-7"},{"key":"e_1_2_5_4_1","first-page":"215","article-title":"Cryptorchidism. Semin. Pediatr","volume":"19","author":"Hutson JM","year":"2010","journal-title":"Surg"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-004-1290-8"},{"key":"e_1_2_5_6_1","first-page":"e18","article-title":"Gene expression profiling and qRT\u2010PCR expression of RRP1B, PCNT, KIF21A and ADRB2 in leucocytes of Down's syndrome subjects","volume":"91","author":"Salemi M","year":"2012","journal-title":"J Genet"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-7-24"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02290677"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1112915"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod64.6.1583"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10072-012-1152-4"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"(2001) Analysis of relative gene expression data using real\u2010time quantitative PCR and the 2(\u2010Delta Delta C(T))","volume":"4","author":"Livak KJ","year":"2001","journal-title":"Methods"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5472.1822"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133927"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.6.2464"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1210\/endo.136.2.7835302","article-title":"Stage and cell specific expression of the adenosine 3\u2032\u20105\u2032 monophosphate\u2010phosphodiesterase genes in the rat seminiferous epithelium","volume":"36","author":"Morena AR","year":"1995","journal-title":"Endocrinology"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21835","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21835","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T17:56:02Z","timestamp":1694109362000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21835"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,26]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/jcla.21835"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21835","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"type":"print","value":"0887-8013"},{"type":"electronic","value":"1098-2825"}],"subject":[],"published":{"date-parts":[[2014,12,26]]}}}