{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:08:20Z","timestamp":1761581300370},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,4,14]],"date-time":"2015-04-14T00:00:00Z","timestamp":1428969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["17694"],"award-info":[{"award-number":["17694"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Global reports have highlighted the increasing prevalence of <jats:italic>Candida tropicalis<\/jats:italic> infections as well as organism<jats:sup>\u2019<\/jats:sup>s drug resistance. This study aimed at identifying azole resistance markers in clinical isolates of <jats:italic>C. tropicalis<\/jats:italic>, which will be a great resource for developing new drugs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two susceptible and resistant isolates of <jats:italic>C. tropicalis<\/jats:italic> were recovered from an epidemiological investigation of candidiasis in immunocompromised patients. <jats:italic>C. tropicalis<\/jats:italic> ATCC 750 was used as reference strain. Antifungal susceptibility to fluconazole and itraconazole was determined using Clinical and Laboratory Standards Institute (CLSI) method. Complementary DNA\u2010amplified fragment length polymorphism (cDNA\u2010AFLP) technology and real\u2010time reverse\u2010transcriptase (RT) PCR were used for identification of potential genes involved in azole resistance of <jats:italic>C. tropicalis<\/jats:italic> clinical isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five genes encoding the following enzymes were identified as superoxide dismutase (<jats:italic>SOD<\/jats:italic>) implicated in antioxidant defense, ornithine aminotransferase (<jats:italic>OAT<\/jats:italic>), acetyl ornithine aminotransferase (<jats:italic>ACOAT<\/jats:italic>), adenosylmethionine\u20108\u2010amino\u20107\u2010oxononanoate aminotransferase (<jats:italic>DAPA AT<\/jats:italic>), and 4\u2010aminobutyrate aminotransferase (<jats:italic>ABAT<\/jats:italic>)\u2014belonging to pyridoxal phosphate (PLP) dependent enzymes and acting in an important physiological role in many fungal\u2010cell cycles. Real\u2010time RT\u2010PCR confirmed mRNA level of the aforementioned genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings showed that factors such as PLP\u2010dependent enzymes and <jats:italic>SOD<\/jats:italic> might be implicated in drug resistance in <jats:italic>C. tropicalis<\/jats:italic> clinical isolate. Therefore, further studies are required to explore the accurate biological functions of the mentioned genes that would be helpful for effective drug development.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21847","type":"journal-article","created":{"date-parts":[[2015,4,14]],"date-time":"2015-04-14T16:28:46Z","timestamp":1429028926000},"page":"266-272","source":"Crossref","is-referenced-by-count":5,"title":["Identification of Azole Resistance Markers in Clinical Isolates of <i>Candida tropicalis<\/i> Using cDNA\u2010AFLP Method"],"prefix":"10.1002","volume":"30","author":[{"given":"Ali","family":"Kanani","sequence":"first","affiliation":[{"name":"Department of Medical Mycology and Parasitology School of Public Health, Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farideh","family":"Zaini","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology and Parasitology School of Public Health, Tehran 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