{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:28:38Z","timestamp":1740104918305,"version":"3.37.3"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009576","name":"Research Project of Nantong Health Bureau","doi-asserted-by":"crossref","award":["W201217"],"award-info":[{"award-number":["W201217"]}],"id":[{"id":"10.13039\/501100009576","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Natural Science Research Project of Nantong Science and Technology Bureau","award":["HS2013028","HS2013018"],"award-info":[{"award-number":["HS2013028","HS2013018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatitis B virus (HBV) G1896A mutation was associated with HBeAg seronegativity and hepatitis B related acute\u2010on\u2010chronic liver failure. In this study, we developed Taqman amplification refractory mutation system (Taqman\u2010ARMS) and established a strict control system to detect HBV G1896A mutant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HBV viral DNA was isolated from 60 patient serum samples, and full\u2010length HBV genome was cloned. Then, Taqman\u2010ARMS was used to detect HBV G1896A mutant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The assay has the sensitivity of 1E+3 IU\/ml G1896A template, and 0.1% weak population virus with G1896A could be found in mixtures. Total of all 60 clinical samples random collected were detected by Taqman\u2010ARMS, the results were consistent with those by DNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The proposed Taqman\u2010ARMS real\u2010time PCR method for the detection of G1896A mutation of HBV was rapid, simple, sensitive, specific, and applicable in the clinical setting.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21857","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T07:38:01Z","timestamp":1431502681000},"page":"315-318","source":"Crossref","is-referenced-by-count":1,"title":["Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman\u2010ARMS Followed a Strict Control System"],"prefix":"10.1002","volume":"30","author":[{"given":"Renfei","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Laboratory The Third People's Hospital of Nantong  Nantong, Jiangsu P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Laboratory The Third People's Hospital of Nantong  Nantong, Jiangsu P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhou","sequence":"additional","affiliation":[{"name":"Nantong Center for Disease Control and Prevention  Nantong, Jiangsu P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nantong Center for Disease Control and Prevention  Nantong, Jiangsu P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory The Third People's Hospital of Nantong  Nantong, Jiangsu P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,13]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i48.9189"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084453"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.22159"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2007.01585.x"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90713-7"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-010-0315-4"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-006-1997-5"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.254-258.2005"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.12.6601-6612.2003"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.9.5437-5444.1995"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.05.020"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00045-09"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10736"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.7.2503"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2324"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-6722.2001.00074.x"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/11751"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007746"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21857","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T15:04:44Z","timestamp":1696172684000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21857"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,13]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1002\/jcla.21857"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21857","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"type":"print","value":"0887-8013"},{"type":"electronic","value":"1098-2825"}],"subject":[],"published":{"date-parts":[[2015,5,13]]}}}