{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:07:31Z","timestamp":1777435651962,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,10,10]],"date-time":"2015-10-10T00:00:00Z","timestamp":1444435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Bunkyo Gakuin University Research Fund"},{"name":"Japan Society for the Promotion of Science Grant-in-Aid for Young Scientists (B)","award":["23790627"],"award-info":[{"award-number":["23790627"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Analysis of urinary proteins using cellulose acetate membrane electrophoresis (CAME) is a useful and challenging method for the recognition of damaged sites in the kidney. However, protein content of each CAME fraction is still not completely understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, an effective method of protein extraction from each band fractionated by CAME was established, which enabled us to examine the extracted proteins by sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis and mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Proteins were extracted from the gel and analyzed by mass spectrometry. In all, 31 proteins were identified, including 20 urinary proteins that were newly identified in the CAME\u2010based analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This methodology was useful for identifying the proteins responsible for creating unique bands on CAME in a urine sample of a patient with drug\u2010induced interstitial nephritis. These findings provide in\u2010depth characterization of urinary protein contents in each CAME fraction.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21863","type":"journal-article","created":{"date-parts":[[2015,10,10]],"date-time":"2015-10-10T16:14:22Z","timestamp":1444493662000},"page":"359-367","source":"Crossref","is-referenced-by-count":4,"title":["Cellulose Acetate Membrane Electrophoresis Based Urinary Proteomics for the Identification of Characteristic Proteins"],"prefix":"10.1002","volume":"30","author":[{"given":"Aki","family":"Nakayama","sequence":"first","affiliation":[{"name":"Faculty of Health Science Technology Bunkyo Gakuin University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Kubota","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Saitama Prefectural University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Sakatsume","sequence":"additional","affiliation":[{"name":"Division of Clinical Nephrology and Rheumatology Graduate School of Medical and Dental Sciences, Niigata University  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Graduate School of Medicine, Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Katayama","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoko","family":"Kanamori","sequence":"additional","affiliation":[{"name":"Faculty of Health Science Technology Bunkyo Gakuin University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoko","family":"Shiba","sequence":"additional","affiliation":[{"name":"Division of Health Care Science Graduate School of Health Care Science, Bunkyo Gakuin University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Iijima","sequence":"additional","affiliation":[{"name":"Faculty of Health Science Technology Bunkyo Gakuin University  Tokyo Japan"},{"name":"Division of Health Care Science Graduate School of Health Care Science, Bunkyo Gakuin University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.5301\/jn.5000233"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2013.06.016"},{"key":"e_1_2_6_4_1","first-page":"189","article-title":"An overview of urinary proteomics applications in human diseases","volume":"1","author":"Gopalan G","year":"2010","journal-title":"Int J High Throughput Screen"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e31819e4305"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-14-0035"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.04.024"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2825(1996)10:6<403::AID-JCLA15>3.0.CO;2-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20028"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1797.2006.00739.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20049"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.10065"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2198\/jelectroph.54.13"},{"key":"e_1_2_6_14_1","first-page":"248","article-title":"Tojo N, Shiba K, Semba J. 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