{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:17:05Z","timestamp":1790360225018,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We evaluated the new body fluid module on Sysmex UF1000\u2010i (UF1000i\u2010BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid (CSF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>WBC and RBC counting were compared between UF1000i\u2010BF and Fuchs\u2013Rosenthal counting chamber in 67 CSF samples. This study also included the evaluation of between\u2010day precision, limit of blank (LoB), limit of detection (LoD), functional sensitivity (limit of quantitation, LoQ), carryover and linearity. Diagnostic agreement for differentiation between normal and increased WBC counts (\u22655.0 \u00d7 10<jats:sup>6<\/jats:sup>\/L) was also assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The agreement between UF1000i\u2010BF and manual WBC counts was otpiaml in all CSF samples (<jats:italic>r<\/jats:italic> = 0.99; <jats:italic>y<\/jats:italic> = 1.05<jats:italic>x<\/jats:italic> + 0.09). A modest overestimation was noticed in samples with WBC &lt; 30 \u00d7 10<jats:sup>6<\/jats:sup>\/L (<jats:italic>r<\/jats:italic> = 0.95; <jats:italic>y<\/jats:italic> = 1.21<jats:italic>x<\/jats:italic> \u2212 0.15). A good agreement was observed for RBC counts (<jats:italic>r<\/jats:italic> = 0.98; <jats:italic>y<\/jats:italic> = 1.15<jats:italic>x<\/jats:italic> + 0.55), particularly in samples with RBC \u2265 18 \u00d7 10<jats:sup>6<\/jats:sup>\/L (<jats:italic>r<\/jats:italic> = 0.98; <jats:italic>y<\/jats:italic> = 1.01<jats:italic>x<\/jats:italic> + 8.90). Between\u2010day precision was good, with coefficient of variations (CVs) lower than 7.2% for both WBC and RBC. The LoBs were 0.1 \u00d7 10<jats:sup>6<\/jats:sup> WBC\/L and 1.2 \u00d7 10<jats:sup>6<\/jats:sup> RBC\/L, the LoDs were 0.7 \u00d7 10<jats:sup>6<\/jats:sup> WBC\/L and 5.5 \u00d7 10<jats:sup>6<\/jats:sup> RBC\/L, the LoQs were 2.4 \u00d7 10<jats:sup>6<\/jats:sup> WBC\/L and 18.0 \u00d7 10<jats:sup>6<\/jats:sup> RBC\/L, respectively. Linearity was excellent (<jats:italic>r<\/jats:italic> = 1.00 for both WBC and RBC). Carryover was negligible. Excellent diagnostic agreement was obtained at 4.5 \u00d7 10<jats:sup>6<\/jats:sup> WBC\/L cut\u2010off (sensitivity, 100%; specificity, 97.4%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The UF1000i\u2010BF provides rapid and accurate WBC and RBC counts in clinically relevant values of CSF cells. The use of UF1000i\u2010BF may hence allow to replace routine optical counting, except for samples displaying abnormal WBC counts or abnormal scattergram distribution, for which differential cell counts may still be required.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21866","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T03:17:07Z","timestamp":1440472627000},"page":"381-391","source":"Crossref","is-referenced-by-count":22,"title":["Automated Cerebrospinal Fluid Cell Counts Using the New Body Fluid Mode of Sysmex UF\u20101000i"],"prefix":"10.1002","volume":"30","author":[{"given":"Sabrina","family":"Buoro","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII  Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Apassiti Esposito","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII  Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MariaGrazia","family":"Alessio","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII  Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Crippa","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Chemistry, Hospital Papa Giovanni XXIII  Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cosimo","family":"Ottomano","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Chemistry and Hematology Academic Hospital of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Lippi","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Chemistry and Hematology Academic Hospital of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1249221"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2007.10.001"},{"key":"e_1_2_6_4_1","volume-title":"Body Fluid Analysis for Cellular Composition; Approved Guidelines. 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