{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T13:10:44Z","timestamp":1696425044061},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In recent years, the clinical and biological features governing the clinical course of chronic lymphocytic leukemia (CLL) have been most extensively studied. Human leukocyte antigen\u2010G (HLA\u2010G) allows tumor cells to escape from the antitumor effect of the immune system. Recent studies have shown that various tumor cells show an increased HLA\u2010G expression. Data regarding HLA\u2010G expression in CLL are limited and controversial. The aim of this work is to evaluate flow cytometry study of HLA\u2010G expression on cell surface and assess its relationship with other prognostic factors (CD38, ZAP70, beta 2 microglobulin [\u03b22MG]) in patients with CLL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design and methods<\/jats:title><jats:p>Forty\u2010five newly diagnosed CLL cases. White blood cell count, lymphocyte absolute count, hemoglobin level, platelet count, serum lactate dehydrogenase activity, and serum \u03b22MG level were studied at admission. In each patient, morphologic diagnosis of B\u2010CLL was confirmed by flow cytometry HLA\u2010G, CD38 and ZAP70 expression levels were measured with four\u2010color flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HLA\u2010G positivity ranged between 1% and 12% in CLL patients. A significant correlation was found with CD38, ZAP70, disease stage, and \u03b22MG (<jats:italic>P<\/jats:italic> &lt; 0.001). The off\u2010treatment follow\u2010up period was longer in the HLA\u2010G negative group (<jats:italic>P<\/jats:italic> &lt; 0.022).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, we suggest that, in addition to other prognostic factors, surface HLA\u2010G expression can be considered as an independent prognostic factor. However, our work should be confirmed by further prospective studies, a longer off\u2010treatment follow\u2010up period, and a standardized method.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21868","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T03:15:52Z","timestamp":1440472552000},"page":"399-403","source":"Crossref","is-referenced-by-count":4,"title":["Determination of HLA\u2010G Expression and Evaluation of Its Role as a Prognostic Factor in Chronic Lymphocytic Leukemia"],"prefix":"10.1002","volume":"30","author":[{"given":"Gulsum","family":"Ozet","sequence":"first","affiliation":[{"name":"Department of Haemotology Ankara Numune Education and Research Hospital  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mesude","family":"Falay","sequence":"additional","affiliation":[{"name":"Department of Haemotology Ankara Numune Education and Research Hospital  Ankara 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