{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T14:28:04Z","timestamp":1764944884398},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T00:00:00Z","timestamp":1450051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007209","name":"Thailand Graduate Institute of Science and Technology","doi-asserted-by":"publisher","award":["TGIST 01\u201054\u2010035."],"award-info":[{"award-number":["TGIST 01\u201054\u2010035."]}],"id":[{"id":"10.13039\/501100007209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Magnetic nanoparticles (MNPs) have been widely used in medical diagnostic research. In this work, two technologies, MNPs and polymerase chain reaction (PCR), were combined to increase detection sensitivity and specificity. A novel technique based on the MNPs\u2010PCR enzyme\u2010linked gene assay (MELGA) was developed for detection of the <jats:italic>BCR\/ABL<\/jats:italic> abnormal gene in chronic myelogenous leukemia (CML) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An MNPs\u2010labeled <jats:italic>BCR<\/jats:italic> forward primer and a biotin\u2010labeled <jats:italic>ABL<\/jats:italic> reverse primer were used to specifically amplify the target gene. After magnetic separation, the PCR product bound to MNPs labeled with streptavidin\u2010conjugated horseradish peroxidase was incubated with the peroxidase substrate and hydrogen peroxide to generate the colorimetric signal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When compared with real\u2010time quantitative\u2010PCR (RQ\u2010PCR), the MELGA technique exhibited an increased sensitivity of &lt;1 fg with high specificity for the <jats:italic>BCR\/ABL<\/jats:italic> fusion gene in CML patients. In addition, MELGA colorimetric results correlated well with the number of copies obtained from RQ\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This simple and cost\u2010effective technique is suitable for monitoring CML patients during targeted therapy (tyrosine kinase inhibitors) especially in rural hospitals.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21899","type":"journal-article","created":{"date-parts":[[2015,12,15]],"date-time":"2015-12-15T04:05:39Z","timestamp":1450152339000},"page":"534-542","source":"Crossref","is-referenced-by-count":10,"title":["Magnetic Nanoparticles PCR Enzyme\u2010Linked Gene Assay for Quantitative Detection of <i>BCR\/ABL<\/i> Fusion Gene in Chronic Myelogenous Leukemia"],"prefix":"10.1002","volume":"30","author":[{"given":"Yanaphat","family":"Manthawornsiri","sequence":"first","affiliation":[{"name":"Faculty of Medical Technology Mahidol University  Bangkok 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