{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:42:57Z","timestamp":1770273777737,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T00:00:00Z","timestamp":1457913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30872285"],"award-info":[{"award-number":["30872285"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Chlamydia trachomatis<\/jats:italic> causes the most common bacterial sexually transmitted infection (STI) worldwide. Although highly sensitive nucleic acid amplification tests (NAATs) are used to routinely diagnose chlamydial infection, <jats:italic>C. trachomatis<\/jats:italic> isolation by cell culture is still preferred for legal cases and epidemiological studies because of its high specificity; however, the sensitivity of traditional two\u2010passage diagnostic cultures is significantly lower than that of NAATs. Therefore, we sought to analyze if additional in vitro passaging of clinical samples would improve detection sensitivity of <jats:italic>C. trachomatis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Clinical swabs (<jats:italic>n<\/jats:italic> = 428) were collected from Tianjin Medical University General Hospital, grown in McCoy cells for up to five passages, and analyzed for the presence of inclusions by iodine staining. Results were confirmed by routine PCR\u2010based methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Viable <jats:italic>C. trachomatis<\/jats:italic> organisms were detected in 91 (21.26%) swabs with the traditional two\u2010passage protocol, which increased to 145 (33.88%) and 149 (34.81%) following three and four passages, respectively. Thus, the standard protocol yielded a false\u2010negative rate of nearly 39%. Subsequent PCR\u2010based diagnostics revealed a concordance rate of 80.98% between these two methods without any false negatives.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results of this study support the use of a three\u2010passage <jats:italic>Chlamydia<\/jats:italic> culture procedure to increase the detection sensitivity of this method.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21924","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T11:45:19Z","timestamp":1458301519000},"page":"697-701","source":"Crossref","is-referenced-by-count":18,"title":["Sensitivity of the Standard <i>Chlamydia trachomatis<\/i> Culture Method Is Improved After One Additional In Vitro Passage"],"prefix":"10.1002","volume":"30","author":[{"given":"Lili","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanli","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong","family":"You","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanzhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,14]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"207","volume-title":"Global incidence and prevalence of selected curable sexually transmitted infections: Reproductive Health Matters 2008","author":"Organization WH","year":"2012"},{"key":"e_1_2_5_3_1","unstructured":"Centers for Disease Control and Prevention.Sexually Transmitted Disease Surveillance2012.Atlanta: U.S. Department of Health and Human Services; 2013."},{"issue":"3","key":"e_1_2_5_4_1","first-page":"303","article-title":"Genital Chlamydia trachomatis: An update","volume":"138","author":"Malhotra M","year":"2013","journal-title":"Indian J Med Res"},{"issue":"1","key":"e_1_2_5_5_1","first-page":"29","article-title":"Classical and molecular methods for evaluation of Chlamydia trachomatis infection in women with tubal factor infertility","volume":"14","author":"Hajikhani B","year":"2013","journal-title":"J Reprod Infertil"},{"key":"e_1_2_5_6_1","unstructured":"ByP PappJR SchachterJ GaydosCA PolBVD.Recommendations for the laboratory\u2010based detection ofChlamydia trachomatisandNeisseria gonorrhoeae\u2013 2014.Mmwrrecommendations & Reports Morbidity & Mortality Weekly Reportrecommendations & Reports2014;63(RR\u201002):1\u201319."},{"issue":"5","key":"e_1_2_5_7_1","first-page":"337","article-title":"Efficacy of cytology for the diagnosis of Chlamydia trachomatis in pregnant women","volume":"10","author":"Mda C","year":"2006","journal-title":"Braz J Infect Dis"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2013.01.015"},{"issue":"1","key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1155\/2005\/359046","article-title":"The laboratory diagnosis of Chlamydia trachomatis infections","volume":"16","author":"Chernesky MA.","year":"2005","journal-title":"Can J Infect Dis Med Microbiol"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01301-10"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S46678"},{"issue":"7","key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1016\/j.ophtha.2010.12.004","article-title":"A 13\u2010year retrospective review of polymerase chain reaction testing for infectious agents from ocular samples","volume":"118","author":"Thompson PP","year":"2011","journal-title":"Ophthalmology"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016971"},{"issue":"5","key":"e_1_2_5_14_1","first-page":"337","article-title":"Efficacy of cytology for the diagnosis of Chlamydia trachomatis in pregnant women","volume":"10","author":"Mda C","year":"2006","journal-title":"Braz J Infect Dis"},{"issue":"6","key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1097\/OLQ.0b013e318205e435","article-title":"Evaluation of one\u2010sample testing of self\u2010obtained vaginal swabs and first catch urine samples separately and in combination for the detection of Chlamydia trachomatis by two amplified DNA assays in women visiting a sexually transmitted disease clinic","volume":"38","author":"Dommelen L","year":"2011","journal-title":"Sex Transm Dis"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02595-10"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.2.119"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00509.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/652394"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21924","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21924","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T21:08:32Z","timestamp":1696367312000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21924"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,14]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1002\/jcla.21924"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21924","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,14]]}}}