{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:09:41Z","timestamp":1790086181600,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The General Research Grants from Zhejiang Medicine Health Science and Technology Projects","award":["2013KYA230"],"award-info":[{"award-number":["2013KYA230"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>As the second leading cause of cancer morbidity and death in women, cervical cancer remains an important public health problem worldwide. Novel biomarkers with high sensitivity and specificity for the early detection and diagnosis of cervical cancer are urgently needed. Increasing evidence shows that long noncoding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are differentially expressed in cancer tissues and may serve as diagnostic markers. In multiple tumor types, exosomes harboring lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s are actively released from tumor cells. In this study, we investigate the potential association of exosomal lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression with cervical cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cervicovaginal lavage specimens were collected from patients with cervical cancer and cancer\u2010free volunteers who are <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive or <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010negative. Exosomes in these specimens were isolated by ultracentrifugation and confirmed by transmission electron microscopy. The exosomal lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s <jats:styled-content style=\"fixed-case\">HOTAIR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MALAT<\/jats:styled-content>1, and <jats:styled-content style=\"fixed-case\">MEG<\/jats:styled-content>3 were quantified by <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of <jats:styled-content style=\"fixed-case\">HOTAIR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MALAT<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">MEG<\/jats:styled-content>3 was predominantly observed in cervical cancer\u2010derived exosomes in cervicovaginal lavage samples. The expression levels of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were significantly different in exosomes isolated from cervical cancer patients compared to normal controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data suggest that lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in exosomes isolated from cervicovaginal lavage are differentially expressed in cervical cancer patients and cancer\u2010free volunteers. Exosomal lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s may have great potential to be used for the early detection and diagnosis of cervical cancer, and serve as convenient and noninvasive biomarkers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21990","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T06:03:47Z","timestamp":1463465027000},"page":"1116-1121","source":"Crossref","is-referenced-by-count":127,"title":["Exosomal Long Noncoding <scp>RNA<\/scp>s are Differentially Expressed in the Cervicovaginal Lavage Samples of Cervical Cancer Patients"],"prefix":"10.1002","volume":"30","author":[{"given":"Jin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Taizhou Municipal Hospital Taizhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang\u2010Chun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da\u2010Kang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Taizhou Municipal Hospital Taizhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61684-5"},{"key":"e_1_2_6_3_1","article-title":"LncRNAs: Key players and novel insights into cervical cancer","author":"Peng L","year":"2015","journal-title":"Tumour 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