{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T21:29:39Z","timestamp":1757453379941,"version":"3.41.2"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,2,26]],"date-time":"2017-02-26T00:00:00Z","timestamp":1488067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Sexually transmitted diseases easily spread among sexually active people and often have no symptoms. Rapid and accurate method for detecting these infections are necessary in early stages. The traditional detection methods of them are difficult and time\u2014consuming.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, multiplex real time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>was optimized for rapid identification of<jats:italic>Chlamydia trachomatis<\/jats:italic>and<jats:italic>Mycoplasma hominis<\/jats:italic>in a single tube and was performed with our designed primers. The sensitivity test was carried out to designed primers with diluted genomic<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. To defined the specificity, non<jats:styled-content style=\"fixed-case\">STD<\/jats:styled-content>bacteria were used as<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>template.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This study indicated that the developed multiplex real time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>can be an effective alternative procedure to the conventional methods for rapid and accurate identification of C<jats:italic>Chlamydia trachomatis<\/jats:italic>and<jats:italic>Mycoplasma hominis<\/jats:italic>. Multiplex real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>Results of them were checked with melting curves. The sensitivity of our designed primer by multiplex real time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>for<jats:italic>Chlamydia trachomatis<\/jats:italic>and<jats:italic>Mycoplasma hominis<\/jats:italic>were 4.78\u00d710<jats:sup>10<\/jats:sup>and 8.35\u00d710<jats:sup>10<\/jats:sup>, respectively, Which the primers did not amplify any product from a non\u2010<jats:styled-content style=\"fixed-case\">STD<\/jats:styled-content>species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Multiplex real time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>by our new primers and analysis of melting curves were successfully usable for rapid and accurate detection of<jats:italic>Chlamydia trachomatis<\/jats:italic>and<jats:italic>Mycoplasma hominis<jats:bold>.<\/jats:bold><\/jats:italic>This assay instead of traditional culture method, has considerable potential to be rapid, accurate and highly sensitive molecular diagnostic tool for simultaneous and direct detection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22126","type":"journal-article","created":{"date-parts":[[2017,2,26]],"date-time":"2017-02-26T07:16:44Z","timestamp":1488093404000},"source":"Crossref","is-referenced-by-count":10,"title":["Development a rapid and accurate multiplex real time PCR method for the detection<i>Chlamydia trachomatis<\/i>and<i>Mycoplasma hominis<\/i>"],"prefix":"10.1002","volume":"31","author":[{"given":"Roya","family":"Safarkar","sequence":"first","affiliation":[{"name":"Department of Biology, Science and Research Branch Islamic Azad University Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jalil Fallah","family":"Mehrabadi","sequence":"additional","affiliation":[{"name":"The Lister Laboratory of Microbiology Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Noormohammadi","sequence":"additional","affiliation":[{"name":"Department of 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