{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:45:21Z","timestamp":1777643121517,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T00:00:00Z","timestamp":1484870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004686","name":"Deanship of Scientific Research, King Faisal University","doi-asserted-by":"publisher","award":["project No. RGP\u2010VPP\u2010175"],"award-info":[{"award-number":["project No. RGP\u2010VPP\u2010175"]}],"id":[{"id":"10.13039\/501100004686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The structural perturbations in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> molecule may be caused by a break in a strand, a missing base from the backbone, or a chemically changed base. These alterations in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> that occurs naturally can result from metabolic or hydrolytic processes. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage plays a major role in the mutagenesis, carcinogenesis, aging and various other patho\u2010physiological conditions. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage can be induced through hydrolysis, exposure to reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) and other reactive carbonyl metabolites including 4\u2010hydroxynonenal (<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content>). 4\u2010<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content> is an important lipid peroxidation product which has been implicated in the mutagenesis and carcinogenesis processes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The present study examines to probe the presence of auto\u2010antibodies against 4\u2010hydroxynonenal damaged <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>) in various cancer subjects. In this study, the purified calf thymus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was damaged by the action of 4\u2010<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was incubated with 4\u2010<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content> for 24\u00a0h at 37\u00b0C temperature. The binding characteristics of cancer auto\u2010antibodies were assessed by direct binding and competitive inhibition <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> modifications produced hyperchromicity in <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> spectrum and decreased fluorescence intensity. Cancer sera exhibited enhanced binding with the 4\u2010<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content> modified calf thymus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> as compared to its native conformer. The 4\u2010<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content> modified <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> presents unique epitopes which may be one of the factors for the auto\u2010antibody induction in cancer patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content> modified <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> presents unique epitopes which may be one of the factors for the autoantibody induction in cancer patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22130","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T15:49:32Z","timestamp":1484927372000},"source":"Crossref","is-referenced-by-count":6,"title":["Preferential recognition of auto\u2010antibodies against <i>4\u2010<\/i>hydroxynonenal modified <scp>DNA<\/scp> in the cancer 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