{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:37:04Z","timestamp":1761129424455,"version":"3.41.2"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,3,9]],"date-time":"2017-03-09T00:00:00Z","timestamp":1489017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Anti\u2010tissue transglutaminase (anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content>) and endomysium antibodies (<jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>) are detectable in duodenal culture media of celiac disease (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>) patients. To improve the management of this organ culture system, we evaluated the anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> occurrence by immunochromatographic assay (<jats:styled-content style=\"fixed-case\">ICA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 103 <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients and 41 disease controls underwent duodenal biopsy for the organ culture. In culture supernatants, IgA anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> were tested by both enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">ICA<\/jats:styled-content>, IgA <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> were searched by indirect immunofluorescence analysis (<jats:styled-content style=\"fixed-case\">iIFA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Endomysium antibodies and anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> measured by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> were positive in culture media of all <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients, while anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> detected by <jats:styled-content style=\"fixed-case\">ICA<\/jats:styled-content> were positive in culture media of 87\/103 <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients. Anti\u2010<jats:styled-content style=\"fixed-case\">tTG ICA<\/jats:styled-content> scores significantly correlated with anti\u2010<jats:styled-content style=\"fixed-case\">tTG ELISA<\/jats:styled-content> values (<jats:italic>r<\/jats:italic>=.71, <jats:italic>P<\/jats:italic>&lt;.0001). Sensitivity, specificity and diagnostic accuracy of anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> detected by <jats:styled-content style=\"fixed-case\">ICA<\/jats:styled-content> were 84.5%, 100% and 88.9%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Using <jats:styled-content style=\"fixed-case\">ICA<\/jats:styled-content>, anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> are detectable in duodenal culture media of most <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients and the intensity of indicative lines depends on the anti\u2010<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content> concentration. Sensitivity and diagnostic accuracy achieved with <jats:styled-content style=\"fixed-case\">ICA<\/jats:styled-content> are lower than those obtained with <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> but, given that the first is a more easy and prompt method, data suggest the possibility of utilizing it in the in vitro diagnosis of CD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22195","type":"journal-article","created":{"date-parts":[[2017,3,9]],"date-time":"2017-03-09T11:05:49Z","timestamp":1489057549000},"source":"Crossref","is-referenced-by-count":3,"title":["One\u2010step immunochromatographic visual assay for anti\u2010transglutaminase detection in organ culture system: An easy and prompt method to simplify the in vitro diagnosis of celiac disease"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7111-0974","authenticated-orcid":false,"given":"Marco","family":"Di Tola","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties Sapienza University  Rome Italy"},{"name":"Department of Biomedical Services\u2014UOC Laboratory Analysis Marsica\/Peligno\/Sangrina Area ASL 1 Avezzano Sulmona L'Aquila  Avezzano AQ Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariacatia","family":"Marino","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties Sapienza University  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossella","family":"Casale","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties Sapienza University  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaele","family":"Borghini","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties Sapienza University  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Tiberti","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties Sapienza University  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Donato","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Sapienza University  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umberto","family":"Occhiuzzi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Services\u2014UOC Laboratory Analysis Marsica\/Peligno\/Sangrina Area ASL 1 Avezzano Sulmona L'Aquila  Avezzano AQ Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Picarelli","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties Sapienza University  Rome 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