{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:25Z","timestamp":1753869385160,"version":"3.41.2"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T00:00:00Z","timestamp":1489968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tianjin Centers for Disease Control and Prevention","award":["CDCKY1503"],"award-info":[{"award-number":["CDCKY1503"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Ofloxacin (<jats:styled-content style=\"fixed-case\">OFX<\/jats:styled-content>) resistant <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content>) isolates have been increasingly observed and are a major concern in recent years. This study investigated the genetic mutations associated with <jats:styled-content style=\"fixed-case\">OFX<\/jats:styled-content> resistance among clinical <jats:styled-content style=\"fixed-case\">OFX<\/jats:styled-content> mono\u2010resistant <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> isolates from new and previously treated tuberculosis patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 50 unrelated <jats:styled-content style=\"fixed-case\">OFX<\/jats:styled-content> mono\u2010resistant <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> isolates were analyzed. For all isolates, the quinolone resistance determining regions of <jats:italic>gyrA<\/jats:italic> and <jats:italic>gyrB<\/jats:italic> were <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> amplified and sequenced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Single mutations in the quinolone resistance determining regions of <jats:italic>gyrA<\/jats:italic> (positions D94A, G, N, and Y; A90V; and S91P) and <jats:italic>gyrB<\/jats:italic> (positions T539A and E540D) were observed in 62% (31\/50) and 4% (2\/50) of all <jats:styled-content style=\"fixed-case\">OFX<\/jats:styled-content> mono\u2010resistant isolates, respectively. No differences were detected between the proportions of isolates with mutations in <jats:italic>gyrA<\/jats:italic>\/<jats:italic>gyrB<\/jats:italic> from new and previously treated tuberculosis patients (<jats:italic>P<\/jats:italic>=.820).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although mutations in <jats:italic>gyrB<\/jats:italic> were rare, they were as important as mutations in <jats:italic>gyrA<\/jats:italic> in predicting <jats:styled-content style=\"fixed-case\">OFX<\/jats:styled-content> resistance in <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> in Tianjin, China.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22202","type":"journal-article","created":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T06:46:58Z","timestamp":1489992418000},"source":"Crossref","is-referenced-by-count":2,"title":["Molecular characteristics of ofloxacin mono\u2010resistant <i>Mycobacterium tuberculosis<\/i> isolates from new and previously treated tuberculosis patients"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5860-5462","authenticated-orcid":false,"given":"Zhirui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Microbiology Tianjin Centers for Disease Control and Prevention  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Microbiology Tianjin Centers for Disease Control and Prevention  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Mu","sequence":"additional","affiliation":[{"name":"Tuberculosis Reference Laboratory Tianjin Center for Tuberculosis Control  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Tuberculosis Reference Laboratory Tianjin Center for Tuberculosis Control  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanfang","family":"Ju","sequence":"additional","affiliation":[{"name":"Tuberculosis Reference Laboratory Tianjin Center for Tuberculosis Control  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tuberculosis Reference Laboratory Tianjin Center for Tuberculosis Control  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Sun","sequence":"additional","affiliation":[{"name":"Tuberculosis Reference Laboratory Tianjin Center for Tuberculosis Control  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,20]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"World Health Organization.Global tuberculosis 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