{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:13:37Z","timestamp":1773789217045,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T00:00:00Z","timestamp":1492041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>To explore the effects of HbJ Bangkok, HbE, HbG Taipei, and \u03b1\u2010thalassemia HbH on the results of HbA1c assessment using ion\u2010exchange high\u2010performance liquid chromatography (<jats:styled-content style=\"fixed-case\">IE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled five patients in which the results of the <jats:styled-content style=\"fixed-case\">IE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> HbA1c assay were inconsistent with the average levels of <jats:styled-content style=\"fixed-case\">FBG<\/jats:styled-content>. We performed hemoglobin capillary (Hb) electrophoresis using whole\u2010blood samples. We also sequenced the genes encoding Hb using dideoxy\u2010mediated chain termination and analyzed HbA1c using borate affinity <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>) and turbidimetric inhibition immunoassay (<jats:styled-content style=\"fixed-case\">TINIA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two patients had the HbJ Bangkok variant. Hb genotypes of these patients were \u03b2<jats:sup>41\u201042<\/jats:sup>\/\u03b2<jats:sup>J Bangkok<\/jats:sup> and \u03b2<jats:sup>N<\/jats:sup>\/\u03b2<jats:sup>J Bangkok<\/jats:sup>, and the content of HbJ Bangkok was 93.9% and 52.4%, respectively. The remaining three patients had the following: HbE (\u03b2<jats:sup>N<\/jats:sup>\/\u03b2<jats:sup>E<\/jats:sup> Hb genotype, 23.6% HbE content), HbG Taipei (\u03b2<jats:sup>N<\/jats:sup>\/\u03b2<jats:sup>G Taipei<\/jats:sup> Hb genotype, 39.4% HbG Taipei content), and \u03b1\u2010thalassemia HbH (6.1% HbH content, 2.8% Hb Bart's content). In the patients with \u03b2\u2010thalassemia and HbJ Bangkok variants, the presence of the variants interfered with the results of HbA1c analyses using <jats:styled-content style=\"fixed-case\">IE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TINIA<\/jats:styled-content>; in the remaining four patients, there was interference with the results of HbA1c <jats:styled-content style=\"fixed-case\">IE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> but not with the <jats:styled-content style=\"fixed-case\">TINIA<\/jats:styled-content> assay. There was no interference with <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> HbA1c results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HbJ Bangkok, HbE, HbG Taipei Hb, and \u03b1\u2010thalassemia HbH disease cause varying degrees of interference with the analysis of HbA1c using <jats:styled-content style=\"fixed-case\">IE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>. In these patients, we suggest using methods free from such interference for the analysis of HbA1c and other indicators to monitor blood glucose levels.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22214","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T18:25:59Z","timestamp":1492107959000},"source":"Crossref","is-referenced-by-count":3,"title":["Effects of hemoglobin variants HbJ Bangkok, HbE, HbG Taipei, and HbH on analysis of glycated hemoglobin via ion\u2010exchange high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0700-5272","authenticated-orcid":false,"given":"Xiu\u2010Ming","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory Medicine Centre Zhongshan Hospital Affiliated to Sun Yat\u2010sen University  Zhongshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010Mei","family":"Wen","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Centre Zhongshan Hospital Affiliated to Sun Yat\u2010sen University  Zhongshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng\u2010Nan","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Centre Zhongshan Hospital Affiliated to Sun Yat\u2010sen University  Zhongshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Huan","family":"Suo","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Centre Zhongshan Hospital Affiliated to Sun Yat\u2010sen University  Zhongshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Qiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Centre Zhongshan Hospital Affiliated to Sun Yat\u2010sen University  Zhongshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc14-S014"},{"key":"e_1_2_5_3_1","unstructured":"World Health Organization.Use of Glycated Haemoglobin (HbA1c) in the Diagnosis of Diabetes Mellitus: Abbreviated Report of a WHO Consultation 2011."},{"key":"e_1_2_5_4_1","unstructured":"Diabetes Federation Clinical Guidelines Task Force.Global Guideline for Type 2 Diabetes 2012.http:\/\/www.idf.org\/site\/default\/files\/IDF-Guideline-for-Type-2-Diabetes.pdf. 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