{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:52:38Z","timestamp":1776127958628,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T00:00:00Z","timestamp":1491264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Colorectal cancer (<jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>) has a high morbidity and mortality. Many studies reported that mir\u2010375 is frequently down\u2010regulated in many cancers including esophageal cancer, hepatocellular carcinoma, breast cancer and leukemias.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>Our aim was to study the expression of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010375 and its target gene <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>\u20107 polymorphisms (rs4939827) in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> patients in comparison to control subjects and to correlate these results with clinical data of patients to elucidate their role in pathogenesis and early diagnosis of <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>The present study was conducted on 122 subjects divided into 86 patients with <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> and 36 age\u2010 and sex\u2010matched controls. The followings were done to all subjects: full history taking, full clinical examination, complete blood picture, serum (<jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> AST<\/jats:styled-content>), serum albumin, <jats:styled-content style=\"fixed-case\">CEA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> TLC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PLT<\/jats:styled-content>, and creatinine. Gene expression of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010375 from serum was done by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Gene polymorphism <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s of <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>7 (rs4939827) was also done in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extracted from blood by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As regards the polymorphism of <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>7, we found that <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content> (wild) genotype has high percentage in controls compared to <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cases (36.1% vs 15.1%). Meanwhile, the mutant and heterozygotes genotypes showed high percentage among cases compared to controls (33.7%, and 51.2% respectively) vs (22.2%, and 41.7% respectively) with no significant statistical analysis. There was a statistically significant high T\u2010allelic frequency among cases and C\u2010allelic frequency among controls. There was a statistically significant association between fold change in micro <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (\u2010375) and the susceptibility to <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> as there is down\u2010regulation of the micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010375 in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> group with fold change in 0.42\u00b10.27.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Micro <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010375 and rs4939827 <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>7 could be considered as potential markers for detecting and early diagnosing <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22215","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T16:27:41Z","timestamp":1491409661000},"source":"Crossref","is-referenced-by-count":15,"title":["Impact of micro<scp>RNA<\/scp>\u2010375 and its target gene <scp>SMAD<\/scp>\u20107 polymorphism on susceptibility of colorectal cancer"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3031-3599","authenticated-orcid":false,"given":"Olfat Gamil","family":"Shaker","sequence":"first","affiliation":[{"name":"Biochemistry and Molecular Biology Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shereen Rashad","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Departments of Medical Biochemistry and Molecular Biology Faculty of Medicine Fayoum University  Al Fayoum 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