{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T03:00:47Z","timestamp":1769310047401,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T00:00:00Z","timestamp":1491436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Functional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T\/B\u2010cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (<jats:styled-content style=\"fixed-case\">CFSE<\/jats:styled-content>)\u2010based assay for the assessment of mitogenic T\u2010cell proliferation responses in primary immunodeficiency (<jats:styled-content style=\"fixed-case\">PID<\/jats:styled-content>) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T\u2010cell defects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Unclassified patients (n=46) and controls (n=25) were evaluated for T\u2010cell disorders with <jats:styled-content style=\"fixed-case\">CFSE<\/jats:styled-content>\u2010based assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD3<jats:sup>+<\/jats:sup> blast cells after <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content>\u2010L stimulation were significantly lower in patients (31.1\u00b128.8) than controls (67.9\u00b18.79; <jats:italic>P<\/jats:italic>&lt;.001). Nine patients with low and four patients with normal <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3 values had severely decreased blastic transformation. The proliferation response decreased mostly in combined immunodeficiency group. Sixteen of them had impaired proliferation responses. Appropriate molecular genetical analyses were planned after thorough evaluation of each patient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In vitro lymphocyte cell proliferation analysis by <jats:styled-content style=\"fixed-case\">CFSE<\/jats:styled-content> method is a reliable and practical choice for the assessment of mitogenic T lymphocyte responses in yet unclassified <jats:styled-content style=\"fixed-case\">PID<\/jats:styled-content> patients for targeting further genetical analyses.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22216","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T10:48:42Z","timestamp":1491475722000},"source":"Crossref","is-referenced-by-count":9,"title":["In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary 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