{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:56:53Z","timestamp":1790060213807,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T00:00:00Z","timestamp":1491264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A considerable number of<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>alleles responsible for weak and partial D phenotypes have been identified. Serologic determination of these phenotypes is often doubtful and makes genetic analysis of<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>gene highly desirable in transfusion recipients and pregnant women. We analyzed the<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>gene in a cohort of pregnant women with doubtful D phenotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>genotyping was performed on 104 cases with D typing discrepancies or with history of serologic weak D phenotype. Laboratory\u2010developed<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>tests,<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>BeadChip (Bioarray Solutions, Immucor), and sequencing were used to identify the<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>alleles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Molecular analyses showed 23 of 104 (22%) pregnant women were<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content>*weak D types 1<\/jats:italic>,<jats:italic>2<\/jats:italic>, or<jats:italic>3<\/jats:italic>and not at risk for anti\u2010D. Fifty\u2010one (49%) were<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content>*weak partial 4.0,<\/jats:italic>6<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content>*weak D type 38<\/jats:italic>(6%), 1<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content>*weak D type 45<\/jats:italic>(1%), 1<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content>*weak D type<\/jats:italic>67 (1%), and potentially at risk for being alloimmunized and making anti\u2010D. Partial D was identified in 22 of 104 (21%) patients and definitively at risk for anti\u2010D.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Appropriate classification of RhD phenotypes is recommended for correct indication of Rh<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>in pregnant women. However, the serologic distinction between RhD\u2010negative and RhD\u2010positive phenotypes is a difficult task in the case of D variants due to the variations in serologic testing. Our results show a great variability in<jats:italic><jats:styled-content style=\"fixed-case\">RHD<\/jats:styled-content><\/jats:italic>variant alleles in pregnant women from this population of high admixture. According to these results, 78% of these obstetric patients are at risk for anti\u2010D and candidates for Rh<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22221","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T16:28:05Z","timestamp":1491409685000},"source":"Crossref","is-referenced-by-count":8,"title":["<i><scp>RHD<\/scp><\/i>alleles among pregnant women with serologic discrepant weak D phenotypes from a multiethnic population and risk of alloimmunization"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5103-4970","authenticated-orcid":false,"given":"Carolina Bonet","family":"Bub","sequence":"first","affiliation":[{"name":"Hemotherapy and Cellular Therapy Department Hospital Israelita Albert Einstein Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Giselda","family":"Aravechia","sequence":"additional","affiliation":[{"name":"Hemotherapy and Cellular Therapy Department Hospital Israelita Albert Einstein Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Henrique","family":"Costa","sequence":"additional","affiliation":[{"name":"Hemotherapy and Cellular Therapy Department Hospital Israelita Albert Einstein Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mauro","family":"Kutner","sequence":"additional","affiliation":[{"name":"Hemotherapy and Cellular Therapy Department Hospital Israelita Albert Einstein Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian","family":"Castilho","sequence":"additional","affiliation":[{"name":"Hemotherapy and Cellular Therapy Department Hospital Israelita Albert Einstein Sao Paulo Brazil"},{"name":"Hemocentro Unicamp Campinas 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