{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:30:32Z","timestamp":1785796232839,"version":"3.56.0"},"reference-count":67,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T00:00:00Z","timestamp":1491782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tunisian Ministry of Higher Education, Scientific Research and Technology"},{"name":"Tunisian Ministry of Health","award":["LR12SP11"],"award-info":[{"award-number":["LR12SP11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Adipose tissue is an important endocrine organ that secretes a number of adipokines, like Resistin (<jats:styled-content style=\"fixed-case\">RETN<\/jats:styled-content>); it's an adipocytes\u2010secreted cytokine and has been proposed as a link between obesity and diabetes. Many resistin gene polymorphisms were described and their implication in obesity was controversial. This study was to investigate the prevalence of single nucleotide polymorphisms (<jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s) in<jats:styled-content style=\"fixed-case\">RETN<\/jats:styled-content>gene 420C\/G; 44G\/A; 62G\/A; 394C\/G and 299 G\/A and their association with Resistin level and obesity in Tunisian volunteers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We recruited 169 nonobese (mean age=42.16\u201014.26\u00a0years; mean body mass index [<jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content>]=24.51\u20103.69\u00a0kg\/m<jats:sup>2<\/jats:sup>) and 160 obese (mean age=47.86\u201011.17\u00a0years; mean<jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content>=36\u20104.78\u00a0kg\/m<jats:sup>2<\/jats:sup>). Genotyping was performed using polymerase chain reaction\u2013restriction fragment length polymorphism. Anthropometric parameters, lipid levels, Glycemia and insulinemia were measured,<jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content>was calculated and insulinresistance was evaluated with the homeostasis model assessment insulin resistance (<jats:styled-content style=\"fixed-case\">HOMA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) and resistin level was measured by<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Statistical analyses were performed by<jats:styled-content style=\"fixed-case\">SPSS<\/jats:styled-content>19.0.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After adjustment for confounding parameters; the Odds Ratio (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>) of obesity associated with mutated genotypes at 420C\/G compared with normal genotype was as:<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>=2.17; 95%<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>[1.28\u20103.68],<jats:italic>P<\/jats:italic>=.004. The serum Resistin levels present no significant association with all<jats:styled-content style=\"fixed-case\">RETN<\/jats:styled-content>polymorphisms and it was significantly associated with<jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content>(<jats:italic>P<\/jats:italic>=.047). In our haplotype analysis, one haplotype seems to be protective and one other seems to be the highest risk to obesity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The 420 C\/G Polymorphism were associated with obesity and Leptin concentration in our population.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22227","type":"journal-article","created":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T07:36:51Z","timestamp":1491809811000},"source":"Crossref","is-referenced-by-count":16,"title":["Resistin polymorphims, plasma resistin levels and obesity in Tunisian volunteers"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5610-1068","authenticated-orcid":false,"given":"Nesrine","family":"Zayani","sequence":"first","affiliation":[{"name":"Biochemistry Department Sahloul University Hospital Sousse 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