{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:56:31Z","timestamp":1789052191445,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T00:00:00Z","timestamp":1499040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The 12th Five-Year Plan for National Science and Technology Major Projects foundation","award":["2015ZX10003003"],"award-info":[{"award-number":["2015ZX10003003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>T\u2010cell Xtend (<jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content>) was introduced to extend the blood storage time for T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> test, a widely used commercial interferon gamma release assay (<jats:styled-content style=\"fixed-case\">IGRA<\/jats:styled-content>) for rapid in vitro tuberculosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 99 Uyghur suspected tuberculosis patients were recruited in this study. T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> test was performed with fresh blood (controls), 36\u00a0hours delayed blood and delayed and <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content>\u2010treated (at 36\u00a0hours) blood from each patient, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>White blood cells and lymphocytes proportion in peripheral blood mononuclear cells s and spot\u2010forming cells in positive control wells decreased significantly in delayed blood samples when compared with controls, while this decrease was not detected in <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content>\u2010treated group. In the 58 patients with paired T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> results of three groups of samples, a higher positive rate was observed in <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content>\u2010treated group than both in controls and untreated group (41.4% vs 37.9% and 25.9%). The concordance of T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> results between the treated group and controls was 0.856, whereas the agreement between controls and untreated group was unsatisfactory (0.649). In the 23 elderly patients (&gt;70 years old) with paired T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> results of controls and <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content> group, treated group showed a non\u2010significant trend toward higher positive rate than controls (43.5% vs 26.1%, <jats:italic>P<\/jats:italic>=.22). Meanwhile, <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content> treatment reduced the risk of false negative T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> results in the elderly population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Deterioration of blood sample caused by long storage time can be neutralized by <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content> treatment. The results provide data for the utility of <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content> in a novel population and in Asian region, and reveal the potential of <jats:styled-content style=\"fixed-case\">TCX<\/jats:styled-content> to improve the accuracy of T\u2010<jats:styled-content style=\"fixed-case\">SPOT<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> test in elderly population.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22253","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T05:22:37Z","timestamp":1499059357000},"source":"Crossref","is-referenced-by-count":4,"title":["The performance of T\u2010cell Xtend reagent in increasing blood storage times for interferon gamma release assays"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5681-1203","authenticated-orcid":false,"given":"Junlian","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyamu","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libike","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiying","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenping","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Xinjiang Uyghur Autonomous Region  Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00602-06"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-107"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032652"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2012.00972.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.05.013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1586\/17476348.2.2.253"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"1944","DOI":"10.1128\/JCM.02265-05","article-title":"An in\u2010house RD1\u2010based enzyme\u2010linked immunospot\u2010gamma interferon assay instead of the tuberculin skin test for diagnosis of latent Mycobacterium tuberculosis infection","volume":"44","author":"Codecasa L","year":"2006","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_9_1","first-page":"960","article-title":"Tuberculosis epidemiological sampling survey in Xinjiang Uyghur Autonomous Region (2010\u20102011)","volume":"35","author":"Yang J","year":"2013","journal-title":"Chin J Antituberc"},{"key":"e_1_2_7_10_1","unstructured":"Analysis of prevalence and epidemic trend of lung tuberculosis in Xinjiang Uyghur Autonomous Region (2004\u20102014). Lanzhou University 2015."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023806"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"752071","DOI":"10.1155\/2013\/752071","article-title":"Influence of physical activity and nutrition on obesity\u2010related immune function","volume":"2013","author":"Huang CJ","year":"2013","journal-title":"Sci World J"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2011.10.017"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00674-12"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2014.12.018"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00226613"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1183\/09031936.00179310","article-title":"Influence of pre\u2010analytic conditions on the rate of indeterminate T\u2010SPOT(R).TB tests","volume":"38","author":"Zellweger A","year":"2011","journal-title":"Eur Respir J"},{"key":"e_1_2_7_18_1","first-page":"345290","article-title":"Maintenance of sensitivity of the T\u2010SPOT.TB assay after overnight storage of blood samples, Dar es Salaam, Tanzania","volume":"2012","author":"Talbot EA","year":"2012","journal-title":"Tuberc Res Treat"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.jinf.2010.01.012","article-title":"Comparison of same day versus delayed enumeration of TB\u2010specific T cell responses","volume":"60","author":"Lenders LM","year":"2010","journal-title":"J Infect"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2010.498021"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjhh.2014.03.021"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2011.09.008"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1186\/1471-2334-13-513","article-title":"Poor agreement between interferon\u2010gamma release assays and the tuberculin skin test among HIV\u2010infected individuals in the country of Georgia","volume":"13","author":"Chkhartishvili N","year":"2013","journal-title":"BMC Infect Dis"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2010.0062"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2014.885657"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T20:14:57Z","timestamp":1695759297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,3]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/jcla.22253"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22253","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,3]]},"article-number":"e22253"}}