{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T08:36:43Z","timestamp":1778661403787,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T00:00:00Z","timestamp":1506556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003758","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003758","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,5]]},"abstract":"Background<\/jats:title>New Delhi Metallo\u2010b\u2010lactamase (NDM<\/jats:styled-content>\u20101) is an enzyme emerging around the world conferring resistance to a wide range of \u03b2\u2010lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the bla<\/jats:italic>NDM<\/jats:sub><\/jats:styled-content>\u20101<\/jats:sub> gene using the LOOP<\/jats:styled-content>\u2010mediated isothermal amplification technique (LAMP<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>In all, 14 Gram\u2010negative bacterial strains isolated from patients presenting pneumonia associated with mechanical ventilation were used for the bla<\/jats:italic>NDM<\/jats:sub><\/jats:styled-content>\u20101<\/jats:sub> standardization by LAMP<\/jats:styled-content>. Klebsiella pneumoniae <\/jats:italic>ATCC BAA<\/jats:styled-content>\u20102473 and two clinical strains were used as a positive control. All results were compared to the reaction in polymerase chain reaction (PCR<\/jats:styled-content>), considered gold standard for this detection.<\/jats:p><\/jats:sec>Results<\/jats:title>There was an excellent correlation between the two techniques employed, since all measured clinical strains were negative in both employed tests and two clinical, and a reference strains were positive.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The lamp technique seems to be an excellent option for the rapid detection of bla<\/jats:italic>NDM<\/jats:sub><\/jats:styled-content>\u20101.<\/jats:sub> The amplification time is much shorter than other molecular techniques, the PCR<\/jats:styled-content> machine is not necessary, it is easy of implementation and costs is low.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22323","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T16:16:59Z","timestamp":1506615419000},"source":"Crossref","is-referenced-by-count":8,"title":["Rapid detection of the New Delhi metallo\u2010b\u2010lactamase 1 (NDM<\/scp>\u20101) gene by loop\u2010mediated isothermal amplification (LAMP<\/scp>)"],"prefix":"10.1002","volume":"32","author":[{"given":"Mirna Giselle","family":"Moreira","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidia Miranda","family":"Barreto","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Faculdade de Medicina\/UFMG Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera L\u00facia","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea Souza","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia e Biologia Centro Universit\u00e1rio do Maranh\u00e3o S\u00e3o Lu\u00eds Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vandack","family":"Nobre","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Infectologia e Medicina Tropical Faculdade de Medicina Universidade Federal de Minas Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3777-853X","authenticated-orcid":false,"given":"Simone Gon\u00e7alves","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00774-09"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70279-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2345-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70143-2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-007-0121-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012822"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.5812\/jjm.28831"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01021-06"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3403-3406.2003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1105"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00883-08"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.10.014"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2012.37"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0415"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph110201465"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06199-11"},{"key":"e_1_2_7_19_1","first-page":"1969","article-title":"New Delhi Metallo\u2010\u03b2\u2010Lactamase\u2010mediated Carbapenem resistance: origin, diagnosis, treatment and public health concern","volume":"14","author":"WJ W","year":"2015","journal-title":"Chin Med J (Engl)"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-1012-8"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0063"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2016.08.001"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.03.016"},{"key":"e_1_2_7_24_1","unstructured":"XuZ HouY DanQ.Evaluation of Current Methodologies for Rapid Identification of Methicillin\u2010resistant Phylococcus Aureus Strains. International Conference on Biotechnology and Bioengineering icbb.2016."},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt298"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.01.005"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20144154"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2014.0171"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01458-15"},{"issue":"1","key":"e_1_2_7_30_1","first-page":"31","article-title":"Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on \u03b2\u2010lactams and polymyxins","volume":"47","author":"Sampaio J L M","year":"2016","journal-title":"Br J"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2011.09.005"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22323","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T12:17:04Z","timestamp":1695471424000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,28]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1002\/jcla.22323"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22323","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,28]]},"article-number":"e22323"}}