{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:53:31Z","timestamp":1777575211577,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Poznan University of Medical Sciences - grant for young researchers","award":["502-14-02220351-10256"],"award-info":[{"award-number":["502-14-02220351-10256"]}]},{"name":"Polish Ministry of Science and Higher Education \u201cIuventus Plus\u201d","award":["0127\/IPI\/2015\/73"],"award-info":[{"award-number":["0127\/IPI\/2015\/73"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Technical innovation of autoimmune blistering dermatoses (<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content>s) diagnosis aimed at multiplex approach. Two multiparametric<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>tests are commercially available for<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content>s serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>s and to examine the diagnostic value\/agreement of multivariant<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>in compliance with traditional diagnostic setup for<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, 128 sera from suspected<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content>s patients were studied (27 sera in order to compare<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>s). Multivariant<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>(detection of IgG against desmoglein 1 and 3 \u2010<jats:styled-content style=\"fixed-case\">DSG<\/jats:styled-content>1\/3;<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>180,<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>230, envoplakin, type<jats:styled-content style=\"fixed-case\">VII<\/jats:styled-content>collagen), monovariant<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, and statistical analysis were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>With the use of sera from patients with suspected<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content>s, the multiparametric<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>yield an agreement of 84% with traditional stepwise diagnostics. Multivariant<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>with<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>180 and<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>230 showed 87.5% and 80% sensitivity, 87.5% and 91% specificity, 87.5% reliability as well as 87.5% and 80% positive predictive value, 87.5% and 91% negative predictive value, respectively, in relation to monospecific<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Multivariant<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>with<jats:styled-content style=\"fixed-case\">DSG<\/jats:styled-content>1 and<jats:styled-content style=\"fixed-case\">DSG<\/jats:styled-content>3 showed 50% and 80% sensitivity, 100% and 80% specificity, 85% and 80% reliability as well as 100% and 57% positive predictive value, 82% and 92% negative predictive value, respectively, in relation to monospecific<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. A better rate of agreement was observed among<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>systems with<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>180 and<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>230, than with<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>systems with<jats:styled-content style=\"fixed-case\">DSG<\/jats:styled-content>1 and<jats:styled-content style=\"fixed-case\">DSG<\/jats:styled-content>3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Multivariant<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>test combined with clinical examinations and<jats:styled-content style=\"fixed-case\">DIF<\/jats:styled-content>is recommended as a minimal approach to diagnosing<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content>s in ethnic Slavs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22336","type":"journal-article","created":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T07:26:13Z","timestamp":1506410773000},"source":"Crossref","is-referenced-by-count":9,"title":["Clinical evaluation of a multiparametric<scp>ELISA<\/scp>as a rapid tool for routinely diagnosing IgG\u2010mediated autoimmune blistering dermatoses in ethnic Slavs"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3106-064X","authenticated-orcid":false,"given":"Justyna","family":"Gornowicz\u2010Porowska","sequence":"first","affiliation":[{"name":"Autoimmune Blistering Dermatoses Section Department of Dermatology Poznan University of Medical Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Seraszek\u2010Jaros","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Computational Biology Poznan University of Medical Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Bowszyc\u2010Dmochowska","sequence":"additional","affiliation":[{"name":"Cutaneous Histopathology and Immunopathology Section Department of Dermatology Poznan University of Medical Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawe\u0142","family":"Bartkiewicz","sequence":"additional","affiliation":[{"name":"Autoimmune Blistering Dermatoses Section Department of Dermatology Poznan University of Medical Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El\u017cbieta","family":"Kaczmarek","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Computational Biology Poznan University of Medical Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Dmochowski","sequence":"additional","affiliation":[{"name":"Autoimmune Blistering Dermatoses Section Department of Dermatology Poznan University of Medical Sciences Poznan Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000339776"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2011.606448"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.4103\/ijd.IJD_196_17","article-title":"Advanced diagnostic techniques in autoimmune bullous diseases","volume":"62","author":"Jindal A","year":"2017","journal-title":"Indian J Dermatol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2010.01069.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.07.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/15665240113136660079"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/PLMI.S56781"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.05.007"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-7-49"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.5114\/ada.2017.65617"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.11.002"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2015.2692"},{"key":"e_1_2_6_14_1","unstructured":"MendeM DaehnrichC vanBeekN et\u00a0al.A multivariant profile ELISA for one\u2010step diagnostics of autoimmune bullous dermatoses. 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