{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T07:00:07Z","timestamp":1777532407732,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T00:00:00Z","timestamp":1511395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Our article aims to evaluate the proportion of monospecific antinuclear antibodies (<jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content>) and polyclonal <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content>s in patients with autoimmune diseases based on the results of an <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content> panel and to evaluate the efficiency of trait <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content>s as a novel diagnostic tool. This study also aims to investigate immunoglobulin production in autoimmune diseases by detecting different antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The serum <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content> profile of 634 patients with autoimmune diseases was analyzed using the immunoblot method. A specific formula was developed in an effort to calculate the theoretical proportion of monospecific <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">TPM<\/jats:styled-content>) in different disease groups. Different IgM, IgG, and IgE variants for several pathologies were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The observed proportions of monospecific <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content>s (<jats:styled-content style=\"fixed-case\">OPM<\/jats:styled-content>) were all lower than the predicted <jats:styled-content style=\"fixed-case\">TPM<\/jats:styled-content> in autoimmune diseases. Polyclonal <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content>s were predominant in patients with systemic lupus erythematosus (<jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content>). There were statistical differences in <jats:styled-content style=\"fixed-case\">OPM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TPM<\/jats:styled-content> in all disease groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). Receiver operating characteristic curve (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content> curve) analysis of trait <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content>s between the <jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content> group and the control groups indicated an area under the curve of 0.916. Differences were found in IgM of <jats:italic>Toxoplasma gondii<\/jats:italic> (<jats:styled-content style=\"fixed-case\">TOXO<\/jats:styled-content>) and IgG of hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) and <jats:italic>Treponema pallidum<\/jats:italic> (<jats:styled-content style=\"fixed-case\">TP<\/jats:styled-content>) when comparing the various disease groups to the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The higher <jats:styled-content style=\"fixed-case\">TPM<\/jats:styled-content> suggests that polyclonal differentiation is the major mechanism of <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content> in autoimmune diseases. Trait <jats:styled-content style=\"fixed-case\">ANA<\/jats:styled-content> is potentially a valuable new index for diagnosis in <jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content>. Further investigation is needed to understand the link between B\u2010cell differentiation and autoimmune diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22361","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T10:06:51Z","timestamp":1511431611000},"source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic value of trait antinuclear antibodies and multiple immunoglobulin production in autoimmune diseases"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8679-9719","authenticated-orcid":false,"given":"Liping","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology Dalian Medical University  Dalian Liaoning Province China"},{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Dalian Medical University  Dalian Liaoning Province 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