{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:26Z","timestamp":1753869386857,"version":"3.41.2"},"reference-count":8,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T00:00:00Z","timestamp":1513814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472029","81200224"],"award-info":[{"award-number":["81472029","81200224"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7162155"],"award-info":[{"award-number":["7162155"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,6]]},"abstract":"Introduction<\/jats:title>During routine blood measurements using an automated hematology analyzer, two easily confused types of suspect flags related to lymphocytes often appear: atypical and immature lymphocytes. The aim of this study was to investigate the correlation of high fluorescence cell (HFC<\/jats:styled-content>) parameter and lymphocyte flags determined from an automated hematology analyzer.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 93 patients affected by various pathologic conditions (viral infection, immunological disease, oncological disease and tumor) were divided into an \u201catypical lymphocytes\u201d group (\u201catypical\u201d for short), an \u201cimmature lymphocytes\/blasts\u201d flag group (abnormal), a mixed\u2010flag group that includes \u201catypical lymphocytes\u201d (mixed), and a non\u2010flag group (non\u2010flag).<\/jats:p><\/jats:sec>Results<\/jats:title>The numbers of HFC<\/jats:styled-content>s in the atypical, abnormal, mixed, and non\u2010flag groups were 1.8% (0.9%\u20105.5%), 0.7% (0.1%\u20105.0%), 2.3% (1.2%\u20105.0%), and 0.8% (0.7%\u20101.2%), respectively. The HFC<\/jats:styled-content>s of \u201catypical\u201d appeared as a separate cluster with clear boundaries. The HFC<\/jats:styled-content>s of \u201cabnormal\u201d as an unclear boundaries, and it was difficult to accurately distinguish between the HFC<\/jats:styled-content>s from the immature lymphocytes and the normal lymphocytes. The lower limit of HFC<\/jats:styled-content> when the atypical lymphocyte flag appeared was 0.04\u00a0\u00d7\u00a0109<\/jats:sup>\/L. The number of HFC<\/jats:styled-content>s was similar to atypical lymphocytes detected by microscopy and CD19+<\/jats:sup>CD20\u2212<\/jats:sup>CD27++<\/jats:sup> cells by flow cytometry at 78% and 76%, respectively. The number of HFC<\/jats:styled-content>s detected in \u201catypical\u201d and CD19+<\/jats:sup>CD20\u2212<\/jats:sup>CD27++<\/jats:sup> cells showed good consistency (r<\/jats:italic>\u00a0=\u00a0.715), whereas the consistency was poorest for \u201cabnormal\u201d (r<\/jats:italic>\u00a0=\u00a0.176).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>It demonstrates that HFC<\/jats:styled-content>s reflects atypical lymphocytes better than immature lymphocytes\/blasts.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22374","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T15:03:23Z","timestamp":1513868603000},"source":"Crossref","is-referenced-by-count":2,"title":["Correlation between the cell population in the automated hematology analyzer high\u2010fluorescence region and atypical lymphocyte flags"],"prefix":"10.1002","volume":"32","author":[{"given":"Hongjie","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory National Cancer Center\/Cancer Hospital Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Clinical Laboratory Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory National Cancer Center\/Cancer Hospital Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Clinical Laboratory Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory National Cancer Center\/Cancer Hospital Chinese Academy of Medical Sciences Peking Union Medical College Beijing China"},{"name":"Department of Clinical Laboratory Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2016.792-800"},{"key":"e_1_2_6_3_1","first-page":"ZC01","article-title":"Atypical lymphocytes and cellular cannibalism: a phenomenon, first of its kind to be discovered in chronic periapical lesions","volume":"10","author":"Kalele KP","year":"2016","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_6_4_1","first-page":"1399","article-title":"Are atypical lymphocytes present with viral influenza\u2010like illnesses (ILIs) in hospitalized adults? European journal of clinical microbiology & infectious diseases. official publication of the European Society of Clinical","volume":"35","author":"Cunha BA","year":"2016","journal-title":"Microbiology"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-015-0155-4"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgu222"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20150"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-007-9099-6"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1515\/CCLM.2011.100","article-title":"Sensitivity and specificity of the high fluorescent lymphocyte count\u2010gate on the Sysmex XE\u20105000 hematology analyzer for detection of peripheral plasma cells","volume":"49","author":"Mirre E","year":"2011","journal-title":"Clin Chem Lab Med"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22374","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:22:59Z","timestamp":1695514979000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,21]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/jcla.22374"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22374","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"type":"print","value":"0887-8013"},{"type":"electronic","value":"1098-2825"}],"subject":[],"published":{"date-parts":[[2017,12,21]]},"article-number":"e22374"}}