{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T14:58:54Z","timestamp":1767193134270,"version":"3.41.2"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T00:00:00Z","timestamp":1516233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,6]]},"abstract":"Background<\/jats:title>Alopecia areata (AA<\/jats:styled-content>), which appears as nonscarring hair shedding on any hair\u2010bearing area, is a common organ\u2010specific autoimmune condition. Cytokines have important roles in the development of AA<\/jats:styled-content>. Interleukin (IL<\/jats:styled-content>) 18 is a significant proinflammatory cytokine that was found higher in the patients with AA<\/jats:styled-content>. We aimed to investigate whether the IL<\/jats:styled-content>\u201018 (rs187238 and rs1946518) single nucleotide polymorphisms (SNP<\/jats:styled-content>s) may be associated with AA<\/jats:styled-content> and\/or clinical outcome of patients with AA<\/jats:styled-content> in Turkish population.<\/jats:p><\/jats:sec>Methods<\/jats:title>Genotyping of rs187238 and rs1946518 SNP<\/jats:styled-content>s were detected using sequence\u2010specific primer\u2010polymerase chain reaction (SSP<\/jats:styled-content>\u2010PCR<\/jats:styled-content>) method in 200 patients with AA<\/jats:styled-content> and 200 control subjects.<\/jats:p><\/jats:sec>Results<\/jats:title>The genotype distribution of rs1946518 (\u2212607C>A) SNP<\/jats:styled-content> was found to be statistically significantly different among patients with AA<\/jats:styled-content> and controls (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.0008). Distribution of CC<\/jats:styled-content>+CA<\/jats:styled-content> genotypes and frequency of \u2212607\/allele C of rs1946518 SNP<\/jats:styled-content> were higher in patients with AA<\/jats:styled-content> (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.001, P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.001, respectively). The genotype distribution of rs187238 (\u2212137G>C) SNP<\/jats:styled-content> was found to be statistically significantly different among patients with AA<\/jats:styled-content> and control subjects (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.0014). Distribution of GG<\/jats:styled-content> genotype and frequency of \u2212137\/allele G of rs187238 SNP<\/jats:styled-content> were higher in patients with AA<\/jats:styled-content> (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.0003, P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.001, respectively).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The rs1946518 (\u2212607C>A) and rs187238 (\u2212137G>C) polymorphisms were found associated with alopecia areata disease. The study suggests that IL<\/jats:styled-content>\u201018 rs187238 and rs1946518 SNP<\/jats:styled-content>s may be the cause of the AA<\/jats:styled-content> susceptibility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22386","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T04:52:39Z","timestamp":1516337559000},"source":"Crossref","is-referenced-by-count":13,"title":["Genetic analysis of interleukin 18 gene polymorphisms in alopecia areata"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-5485","authenticated-orcid":false,"given":"Sumeyya Deniz","family":"Celik","sequence":"first","affiliation":[{"name":"Department of Medical Biology Medical Faculty Gaziosmanpasa University Tokat Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omer","family":"Ates","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Medical Faculty Gaziosmanpasa University Tokat Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094260"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2014.910769"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2012.08.014"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.10.032"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12489"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.05.086"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10466.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2009.00771.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208036"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2013.0055"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2012.11.008"},{"key":"e_1_2_6_13_1","first-page":"224","article-title":"IL\u201018 in autoimmunity: review","volume":"17","author":"Boraschi D","year":"2006","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.2007.00714.x"},{"key":"e_1_2_6_15_1","first-page":"1","article-title":"Interleukin\u201018 gene polymorphisms in systemic lupus erythematosus: relation to disease status","volume":"21","author":"Fouad NA","year":"2014","journal-title":"Egypt J Immunol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1399-0039.2003.00137.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-2344-y"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2003.09.032"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(00)00407-0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000351555"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.371"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.129"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/61.7.614"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.260"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07377.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.2152\/jmi.52.236"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062935"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00937.x"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22386","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22386","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:23:51Z","timestamp":1695515031000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,18]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/jcla.22386"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22386","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"type":"print","value":"0887-8013"},{"type":"electronic","value":"1098-2825"}],"subject":[],"published":{"date-parts":[[2018,1,18]]},"article-number":"e22386"}}