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This study was primarily focused on molecular identification of <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content> pathotypes; their association with serogroups and estimates of resistance profiles against different antibiotics regime.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Five hundred seventy\u2010two stool specimens from diarrhea patients were investigated for <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content> pathotypes. Molecular pathotypes were identified by amplification of virulence genes associated with distinct pathotypes followed by sequencing. Diarrhea is a self\u2010limiting disease, however, severity and persistence of infection suggest antibiotic use. Therefore, <jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> were determined against common antibiotic regimen. Correlations between molecular pathotypes and serogroups were analyzed by somatic \u201cO\u201d antigen serotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The present findings reveal incidence of <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content> as an etiological agent up to a level of 21% among all diarrheal age groups. <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content> infection rate was higher in children. Enteropathogenic <jats:italic>E.\u00a0coli<\/jats:italic><jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>, a molecular pathotype of <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content>, was found as a predominant pathotype with highest frequency of 13.7%. Two other molecular pathotypes enterotoxigenic <jats:italic>E.\u00a0coli<\/jats:italic> (<jats:styled-content style=\"fixed-case\">ETEC<\/jats:styled-content>) and enteroaggregative <jats:italic>E.\u00a0coli<\/jats:italic> (<jats:styled-content style=\"fixed-case\">EAEC<\/jats:styled-content>) accounted for 5.7% and 1.3%, respectively for all diarrhea incidences. Serological analysis deciphered somatic antigens O26, O2, and O3 as major serogroups identified among <jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ETEC<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">EAEC<\/jats:styled-content> pathotypes, respectively. All <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content> pathotypes exhibited high levels of antibiotic resistance except for cotrimoxazole and norfloxacin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Comprehensive molecular characterization of <jats:styled-content style=\"fixed-case\">DEC<\/jats:styled-content> pathotypes, their incidence estimates, and antibiogram patterns will help in ascertaining better diagnostic and therapeutic measures in management of diarrheal diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22388","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T15:05:35Z","timestamp":1516633535000},"source":"Crossref","is-referenced-by-count":25,"title":["Molecular characterization of diarrheagenic <i>Escherichia coli<\/i> pathotypes: Association of virulent genes, serogroups, and antibiotic resistance among moderate\u2010to\u2010severe diarrhea patients"],"prefix":"10.1002","volume":"32","author":[{"given":"Nutan","family":"Thakur","sequence":"first","affiliation":[{"name":"Department of Biotechnology &amp; Bioinformatics Jaypee University of Information Technology  Solan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swapnil","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Bioinformatics Jaypee University of Information Technology  Solan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harish","family":"Changotra","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Bioinformatics Jaypee University of Information Technology  Solan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Bioinformatics Jaypee University of Information Technology  Solan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yashwant","family":"Kumar","sequence":"additional","affiliation":[{"name":"National Salmonella &amp; Escherichia coli Centre Central Research Institute  Kasauli India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelam","family":"Grover","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Indira Gandhi Medical College  Shimla India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8702-3170","authenticated-orcid":false,"given":"Jitendraa","family":"Vashistt","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Bioinformatics Jaypee University of Information Technology  Solan India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,21]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Diarrhoeal disease. 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