{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:50:16Z","timestamp":1777459816485,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,2,25]],"date-time":"2018-02-25T00:00:00Z","timestamp":1519516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Study was performed in order: (i) to assess the comparability of glucose, bilirubin, hemoglobin, leukocyte esterase, and protein; (ii) to assess accuracy of glucose, bilirubin, hemoglobin, leukocyte esterase, and protein; and (iii) to evaluate interference of ascorbic acid on the glucose, bilirubin, hemoglobin, and nitrite determination using 2 different dipsticks: <jats:styled-content style=\"fixed-case\">iC<\/jats:styled-content>hem Velocity, Iris Diagnostics and Combur\u201010M, Roche Diagnostics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Random urine specimens were included in the study. Comparability, accuracy, and ascorbic acid interference testing were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Obtained results have shown almost perfect agreement for all parameters between 2 dipsticks in samples with negative ascorbic acid. Agreement in samples with positive ascorbic acid was not acceptable for bilirubin, protein, nitrite, and hemoglobin. Accuracy was not acceptable for hemoglobin and leukocyte esterase on both dipsticks. Ascorbic acid interference examination has shown that intensity of interference differs between dipsticks. Ascorbic acid interferes with glucose, hemoglobin, nitrite, and bilirubin at different concentrations causing false\u2010negative results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Obtained results indicate that it is necessary to determine diagnostic accuracy of used dipstick in order to define purpose of urinalysis. It is very important to choose dipstick with ascorbic acid indicator and to examine ascorbic acid impact on dipstick analytes independently of manufacturer claims.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22390","type":"journal-article","created":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T05:37:49Z","timestamp":1519623469000},"source":"Crossref","is-referenced-by-count":25,"title":["Ascorbic acid\u2014A black hole of urine chemistry screening"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2175-5851","authenticated-orcid":false,"given":"Adriana","family":"Unic","sequence":"first","affiliation":[{"name":"University Department of Chemistry Medical School University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora","family":"Nikolac Gabaj","sequence":"additional","affiliation":[{"name":"University Department of Chemistry Medical School University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0569-924X","authenticated-orcid":false,"given":"Marijana","family":"Miler","sequence":"additional","affiliation":[{"name":"University Department of Chemistry Medical School University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jelena","family":"Culej","sequence":"additional","affiliation":[{"name":"University Department of Chemistry Medical School University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrijana","family":"Lisac","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Laboratory Specialised Hospital for the Extended Medical Treatment  Duga Resa Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Horvat","sequence":"additional","affiliation":[{"name":"University Department of Chemistry Medical School University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nada","family":"Vrkic","sequence":"additional","affiliation":[{"name":"University Department of Chemistry Medical School University Hospital Sestre Milosrdnice  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-711-2_1"},{"key":"e_1_2_7_3_1","first-page":"143","article-title":"Clinical Chemistry Laboratory Automation in the 21st Century\u2014Amat Victoria curam (Victory loves careful preparation)","volume":"35","author":"Armbruster DA","year":"2014","journal-title":"Clin Biochem Rev"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2015.150520"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563216687589"},{"key":"e_1_2_7_6_1","first-page":"1323","article-title":"Accuracy of dipstick urinalysis as a screening method for detection of glucose, protein, nitrites and blood","volume":"15","author":"Zamanzad B","year":"2009","journal-title":"East Mediterr Health J"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2490-4-4"},{"key":"e_1_2_7_8_1","unstructured":"MillanK LizardoS NavarroR et\u00a0al.Impact of Ascorbic Acid interference on the Performance of Urine Chemistry strips: Comparison of Iris iChem STRIPS Arkray AUTION Sticks 9EB Roche URISYS 2400 Cassette and Siemens Atlas Reagent Pack. 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