{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T22:04:51Z","timestamp":1776377091173,"version":"3.51.2"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,17]],"date-time":"2018-02-17T00:00:00Z","timestamp":1518825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The most of the hepatitis C\u2010infected patients remain undiagnosed until they develop severe liver damage or submitted for serological screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate a recombinant multiepitope protein for detection of IgG anti\u2010hepatitis C virus. Method: A synthetic gene was cloned, expressed in <jats:italic>Escherichia coli<\/jats:italic>, and the recombinant protein was purified. Human serum panel consisted of 88 positives (20 <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> genotyped) and 376 negatives for hepatitis C, 6 positives for human acquired immunodeficiency virus, 6 syphilis positives, 6 hepatitis B positives were tested by IgG antihepatitis C virus using the protein by enzyme\u2010linked immunosorbent assay. In addition, 20 positive (all genotyped samples) and 20 negative samples were also tested by immunoblot and dot blot assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Positive hepatitis C sera were strongly reactive against the protein by immunoblot assay. In the dot blot assay, positive sera were reactive until 1:1000 dilution and there were no false positive results in the hepatitis C negative sera. In the enzyme\u2010linked immunosorbent assay, positive and negative sera had significant discrimination. No cross\u2010reaction was observed in samples positive for syphilis; human acquired immunodeficiency virus and hepatitis B. All 20 genotyped samples were positive by the three methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The multiepitope protein used here has a lower cost compared to production of each antigen separately and could be an alternative for the serological diagnosis of hepatitis C.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22410","type":"journal-article","created":{"date-parts":[[2018,2,17]],"date-time":"2018-02-17T06:03:10Z","timestamp":1518847390000},"source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4967-0948","authenticated-orcid":false,"given":"Ronaldo Luis","family":"Thomasini","sequence":"first","affiliation":[{"name":"Faculdade de Medicina de Diamantina (FAMED) NEPii \u2013 N\u00facleo de Estudos de Patologias Infecciosas e Inflamat\u00f3rias Universidade Federal dos Vales do Jequinhonha e Mucuri  Diamantina Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hortencia Gisele Amaro","family":"Souza","sequence":"additional","affiliation":[{"name":"Bioclin\u2010Quibasa  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Bruna\u2010Romero","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia Universidade Federal de Santa Catarina  Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Helvecio","family":"Totola","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Jo\u00e3o Del Rei  Ouro Branco Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neiva Sellan Lopes","family":"Gon\u00e7ales","sequence":"additional","affiliation":[{"name":"Hemocentro Universidade Estadual de Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano Xavier","family":"Lima","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro Martins","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,17]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Guidelines for the screening, care and treatment of persons with hepatitis C infection","author":"WHO","year":"2014"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117742"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11606-015-3209-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27734"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1665-2681(19)30785-9"},{"key":"e_1_2_7_7_1","first-page":"15","article-title":"The hidden epidemic of hepatitis C virus infection in the United States: occult transmission and burden of disease","volume":"21","author":"Ward JW","year":"2013","journal-title":"Top Antivir Med"},{"key":"e_1_2_7_8_1","first-page":"321286","article-title":"Detection of hepatitis C virus coinfection in patients with dengue diagnosis","volume":"000","author":"Machain\u2010Williams C","year":"2014","journal-title":"Biomed Res Int"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ002"},{"key":"e_1_2_7_10_1","first-page":"362","article-title":"Testing for HCV infection: an update of guidance for clinicians and laboratorians","volume":"62","author":"CDC","year":"2013","journal-title":"Morb Mortal Wkly Rep"},{"issue":"5","key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"S106","DOI":"10.4103\/0255-0857.148832","article-title":"Utility of routine real time quantitative PCR monitoring of HCV infection in haemodialysis patients","volume":"33","author":"Datta S","year":"2015","journal-title":"Indian J Med Microbiol"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4314\/ejhs.v24i4.10"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.24078"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)90425-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.12.012"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1044"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i29.9633"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3758\/BRM.41.4.1149"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/2193-1801-2-263"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0407214"},{"key":"e_1_2_7_21_1","first-page":"1","article-title":"A novel structurally stable multiepitope protein for detection of HCV","volume":"000","author":"Galdino AS","year":"2016","journal-title":"Hepat Res Treat"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22410","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T11:54:46Z","timestamp":1695038086000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,17]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1002\/jcla.22410"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22410","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,17]]},"article-number":"e22410"}}