{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:20:56Z","timestamp":1790824856601,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,17]],"date-time":"2018-02-17T00:00:00Z","timestamp":1518825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,7]]},"abstract":"Background<\/jats:title>The von Willebrand factor (VWF<\/jats:styled-content>) multimer test is required to correctly subtype qualitative type 2 von Willebrand disease (VWD<\/jats:styled-content>). The current VWF<\/jats:styled-content> multimer assays are difficult, nonstandardized, and time\u2010consuming. The purpose of this study was to evaluate the clinical utility of the commercial VWF<\/jats:styled-content> multimer kit by Sebia (Lisses, France), an electrophoresis technique yielding same\u2010day results.<\/jats:p><\/jats:sec>Methods<\/jats:title>Ten healthy volunteer plasma samples, in\u2010house reference plasma (IRP<\/jats:styled-content>) and commercial normal plasma (CNP<\/jats:styled-content>) samples, 10 plasma samples from patients with a known VWD<\/jats:styled-content> type, 1 hemophilia A plasma sample, and 7 external quality assurance (EQA<\/jats:styled-content>) samples were analyzed using the commercial VWF<\/jats:styled-content> multimer kit. Additional coagulation testing included measurements of VWF<\/jats:styled-content> antigen (VWF<\/jats:styled-content>:Ag), VWF<\/jats:styled-content> activity (VWF<\/jats:styled-content>:Ac), and FVIII<\/jats:styled-content> activity (FVIII<\/jats:styled-content>:C).<\/jats:p><\/jats:sec>Results<\/jats:title>The CNP<\/jats:styled-content> results revealed a relative loss of the highest molecular weight multimers; therefore, IRP<\/jats:styled-content> was preferred as the reference sample. The interpretations of 10 patients with a known VWD<\/jats:styled-content> type could be successfully reproduced and agreed with previous VWF<\/jats:styled-content> multimer results. In all EQA<\/jats:styled-content> surveys, the multimer results and final VWD<\/jats:styled-content> diagnosis agreed with expert opinion.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The VWF<\/jats:styled-content> multimer assay by Sebia is easy to perform and can be successfully implemented in any clinical laboratory for second\u2010stage evaluation of VWD<\/jats:styled-content>. The resolution power of multimer distribution is adequate to correctly classify VWD<\/jats:styled-content> types 1, 2A, 2B, and 3.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22416","type":"journal-article","created":{"date-parts":[[2018,2,17]],"date-time":"2018-02-17T06:02:59Z","timestamp":1518847379000},"source":"Crossref","is-referenced-by-count":28,"title":["Preclinical evaluation of a semi\u2010automated and rapid commercial electrophoresis assay for von Willebrand factor multimers"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5891-7589","authenticated-orcid":false,"given":"Marika","family":"Pikta","sequence":"first","affiliation":[{"name":"North Estonia Medical Centre Laboratory Tallinn Estonia"},{"name":"Department of Health Technologies School of Information Technologies Tallinn University of Technology Tallinn 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