{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:41Z","timestamp":1753869401514,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:00:00Z","timestamp":1524787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Mevlana University","award":["ICAD:04.09.2015\/150701024"],"award-info":[{"award-number":["ICAD:04.09.2015\/150701024"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Incidence of mycobacterial infections has been increasing. However, diagnosis and treatment of mycobacterial infections can be difficult. The aim of this study was to investigate high\u2010performance liquid chromatography (<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>) analysis of the mycolic acids for rapid identification and dendrogram cluster analysis of mycobacterium species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Clinical specimens received for mycobacterial culture and antimicrobial susceptibility test were processed by standard laboratory protocols. Positive cultures were analyzed with <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> method. Mycolic acid analysis with <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> was used for diagnosis of tuberculosis and other mycobacterial infections. These reports were compared with Sherlock Library mycobacterial species, and the similarity index was analyzed. This value was formed by a software in multidimensional space that was the calculation of the average distance between the nearest library profile and unknown profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The ninety\u2010two samples were identified as <jats:italic>M.\u00a0tuberculosis<\/jats:italic>. (similarity index between 0.593 and 0.994). One of the other strains was identified as <jats:italic>M.\u00a0avium intracellulare<\/jats:italic> (strain No. 82) (<jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>\u00a0=\u00a00.906); one of them was identified as <jats:italic>M.\u00a0interjectum<\/jats:italic> (strain no. 89) (<jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>\u00a0=\u00a00.644). Total 94 samples were identified, and dendrogram was applied to these samples. Profile A (10.6%), profile B (59.6%), profile C (11.7%), profile D (3.2%), and other profiles as single different profiles were identified. Rates for each as 1% (89, 94, 1, 82, 26, 42, 32, 41, 100, 43, 47, 44, 40, 35).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>High\u2010performance liquid chromatography is a useful, rapid, reliable, and practical method for diagnosis of mycobacterium species.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22459","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T11:41:49Z","timestamp":1524829309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Determination of antibiotic resistance and high\u2010performance liquid chromatography profiles for <i>Mycobacterium<\/i> species"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6125-2031","authenticated-orcid":false,"given":"T\u00fcrkan","family":"Toka \u00d6zer","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Konya Hospital  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erkan","family":"Yula","sequence":"additional","affiliation":[{"name":"QMEL Medicine &amp; Publishing Agency  \u0130stanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Metin","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Faculty of Medicine Necmettin Erbakan University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00fcseyin","family":"Bask\u0131n","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Dokuz Eyl\u00fcl University  \u0130zmir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,27]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Global tuberculosis control: surveillance planning financing. 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