{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:29:50Z","timestamp":1780511390948,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,29]],"date-time":"2018-07-29T00:00:00Z","timestamp":1532822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Impaired levels or function of C1 inhibitor (C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content>) results in angioedema due to increased bradykinin. It is important to distinguish between angioedema related to C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> deficiency and that caused by other mechanisms, as treatment options are different. In hereditary (<jats:styled-content style=\"fixed-case\">HAE<\/jats:styled-content>) and acquired (<jats:styled-content style=\"fixed-case\">AAE<\/jats:styled-content>) angioedema, C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> concentration is measured to aid patient diagnosis. Here, we describe an automated turbidimetric assay to measure C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> concentration on the Optilite<jats:sup>\u00ae<\/jats:sup> analyzer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Linearity, precision, and interference were established over a range of C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> concentrations. The 95th percentile reference interval was generated from 120 healthy adult donors. To compare the Optilite C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> assay with a predicate assay used in a clinical laboratory, samples sent for C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> investigation were used. The predicate results were provided to allow comparison.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Optilite C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> assay was linear across the measuring range at the standard sample dilution. Intra and interassay variability was &lt;6%. The 95th percentile adult reference interval for the assay was 0.21\u20100.38\u00a0g\/L. There was a strong correlation between the Optilite concentrations and those generated with the predicate assay (<jats:italic>R<\/jats:italic><jats:sup>2\u00a0<\/jats:sup>=\u00a00.94, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001, slope <jats:italic>y<\/jats:italic>\u00a0=\u00a00.83<jats:italic>x<\/jats:italic>). All patients with Type I <jats:styled-content style=\"fixed-case\">HAE<\/jats:styled-content> (n\u00a0=\u00a024) and <jats:styled-content style=\"fixed-case\">AAE<\/jats:styled-content> (n\u00a0=\u00a03) tested had concentrations below the measuring range in both assays, while all patients with unspecified angioedema (<jats:styled-content style=\"fixed-case\">UAE<\/jats:styled-content>), not diagnosed with <jats:styled-content style=\"fixed-case\">HAE<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">AAE<\/jats:styled-content> had values within the reference range.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The Optilite assay allows the automated and precise quantification of C1\u2010<jats:styled-content style=\"fixed-case\">INH<\/jats:styled-content> concentrations in patient samples. It could therefore be used as a tool to aid the investigation of patients with angioedema.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22627","type":"journal-article","created":{"date-parts":[[2018,7,30]],"date-time":"2018-07-30T06:58:44Z","timestamp":1532933924000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Quantification of human C1 esterase inhibitor protein using an automated turbidimetric immunoassay"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6681-4814","authenticated-orcid":false,"given":"Clare E.","family":"Tange","sequence":"first","affiliation":[{"name":"The Binding Site Group Limited  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrit","family":"Kaur","sequence":"additional","affiliation":[{"name":"The Binding Site Group Limited  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Verma","sequence":"additional","affiliation":[{"name":"Barts Health NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaco","family":"Hickey","sequence":"additional","affiliation":[{"name":"Barts Health NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Grigoriadou","sequence":"additional","affiliation":[{"name":"Barts Health NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Scott","sequence":"additional","affiliation":[{"name":"Barts Health NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorena","family":"Kiani","sequence":"additional","affiliation":[{"name":"Barts Health NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Steven","sequence":"additional","affiliation":[{"name":"Immunodeficiency Centre for Wales University Hospital of Wales  Cardiff UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Ponsford","sequence":"additional","affiliation":[{"name":"Immunodeficiency Centre for Wales University Hospital of Wales  Cardiff UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq","family":"El\u2010Shanawany","sequence":"additional","affiliation":[{"name":"Immunodeficiency Centre for Wales University Hospital of Wales  Cardiff UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Jolles","sequence":"additional","affiliation":[{"name":"Immunodeficiency Centre for Wales University Hospital of Wales  Cardiff UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Harding","sequence":"additional","affiliation":[{"name":"The Binding Site Group Limited  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2434-3660","authenticated-orcid":false,"given":"Antony R.","family":"Parker","sequence":"additional","affiliation":[{"name":"The Binding Site Group Limited  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,29]]},"reference":[{"issue":"1","key":"e_1_2_6_2_1","first-page":"7","article-title":"Overview of hereditary angioedema caused by C1\u2010inhibitor deficiency: assessment and clinical management","volume":"45","author":"Bork K","year":"2013","journal-title":"Eur Ann Allergy Clin Immunol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1710-1492-6-15"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105094"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)09137-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200208223470820"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000368404"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12245-017-0141-z"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.121316"},{"issue":"1","key":"e_1_2_6_10_1","first-page":"24","article-title":"Clinical management of adult patients with a history of nonsteroidal anti\u2010inflammatory drug\u2010induced urticaria\/angioedema: update","volume":"3","author":"Asero R","year":"2007","journal-title":"Allergy Asthma Clin Immunol"},{"issue":"23","key":"e_1_2_6_11_1","first-page":"408","article-title":"Recurrent angioedema and the threat of asphyxiation","volume":"107","author":"Bork K","year":"2010","journal-title":"Dtsch Arztebl Int"},{"issue":"5","key":"e_1_2_6_12_1","first-page":"333","article-title":"Consensus statement on the diagnosis, management, and treatment of angioedema mediated by bradykinin. 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