{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:16:46Z","timestamp":1790439406698,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Background<\/jats:title>Phosphatidylethanols (PE<\/jats:styled-content>ths) are specific, direct alcohol biomarkers that can be determined in human blood to distinguish between heavy and social drinking. PE<\/jats:styled-content>th 16:0\/18:1 is among the most predominant PE<\/jats:styled-content>th homologues in human blood. The aim of the study was to develop a high throughput and sensitive UHPLC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> method for the determination of PE<\/jats:styled-content>th 16:0\/18:1 in whole blood.<\/jats:p><\/jats:sec>Methods<\/jats:title>Whole blood samples were prepared by 96\u2010well supported liquid extraction (SLE<\/jats:styled-content>). Extracted samples were analyzed for PE<\/jats:styled-content>th 16:0\/18:1 by reversed phase UHPLC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>The developed UHPLC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> method was fully validated in whole blood with PE<\/jats:styled-content>th 16:0\/18:1\u2010D5<\/jats:sub> as internal standard. Intermediate precision and intermediate accuracy were within \u2264\u00b1 12% and \u2264\u00b1 17%, respectively, at PE<\/jats:styled-content>th 16:0\/18:1 concentrations of 1.4\u20102112\u00a0ng\/mL<\/jats:styled-content> (2.0\u20103004\u00a0nmol\/L). Limit of quantification (LOQ<\/jats:styled-content>) was 1.7\u00a0ng\/mL<\/jats:styled-content> (2.4\u00a0nmol\/L).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>For the first time, 96\u2010well SLE<\/jats:styled-content> was used for preparation of a PE<\/jats:styled-content>th homologue in biological samples. A mixture of tert\u2010butyl methyl ether and 2\u2010propanol (5:1, v:v) was chosen as organic eluent based on an evaluation of extraction recovery, purity of extracts, and evaporation time. The developed UHPLC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> method can be used for high throughput analyses and sensitive determinations of PE<\/jats:styled-content>th 16:0\/18:1 in whole blood.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22631","type":"journal-article","created":{"date-parts":[[2018,7,26]],"date-time":"2018-07-26T06:33:55Z","timestamp":1532586835000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Determination of phosphatidylethanol 16:0\/18:1 in whole blood by 96\u2010well supported liquid extraction and UHPLC<\/scp>\u2010MS<\/scp>\/MS<\/scp>"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7675-2095","authenticated-orcid":false,"given":"Thomas","family":"Berg","sequence":"first","affiliation":[{"name":"Section of Drug Abuse Research Department of Forensic Sciences Division of Laboratory Medicine Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elin","family":"Eliassen","sequence":"additional","affiliation":[{"name":"Section of Drug Abuse Research Department of Forensic Sciences Division of Laboratory Medicine Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedicte","family":"J\u00f8rgenrud","sequence":"additional","affiliation":[{"name":"Section of Drug Abuse Research Department of Forensic Sciences Division of Laboratory Medicine Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saranda","family":"Kabashi","sequence":"additional","affiliation":[{"name":"Section of Drug Abuse Research Department of Forensic Sciences Division of Laboratory Medicine Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Petukhov","sequence":"additional","affiliation":[{"name":"Moscow Scientific Practical Center of Drug Addiction Moscow Municipal Department of Healthcare Moscow Russia"},{"name":"I.M. 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