{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T19:50:57Z","timestamp":1784663457185,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Temporomandibular disorders (<jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content>) are a group of conditions that cause chronic orofacial pain. The tumor necrosis factor \u03b2 (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2) is a proinflammatory cytokine that is involved in the various aspects of the inflammatory process including organization and maintenance, and in the arrangement of cells at the inflammation site. The purpose of this study was to evaluate the correlation between <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252A\/G (rs909253) variant and susceptibility to <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content> in a Turkish cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 104 patients (26 males, 78 females) with <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content> and 126 healthy controls (44 males, 82 females). The <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252A\/G variant analysis was based on Polymerase Chain Reaction\u2010Restriction Fragment Length Polymorphism (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was no deviation from <jats:styled-content style=\"fixed-case\">HWA<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252A\/G variant in patient and control groups. There was significant difference in genotype and allele frequencies between patient group and control group in terms of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252A\/G variant, respectively (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.010, 0.015). A significant increase in the <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252 <jats:styled-content style=\"fixed-case\">AG<\/jats:styled-content> genotype and G allele frequencies were observed in <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content> patients compared to healthy controls. The individuals with <jats:styled-content style=\"fixed-case\">GG<\/jats:styled-content> genotype and G allele had an increased risk of developing <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content>. A statistically significant association was observed when the patients were compared with the controls according to <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content> genotype vs <jats:styled-content style=\"fixed-case\">AG<\/jats:styled-content>+<jats:styled-content style=\"fixed-case\">GG<\/jats:styled-content> genotypes (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.002, <jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>: 2.23, 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>:1.31\u20103.82). <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252A\/G genotype distribution was associated with chewing problems (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.046).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, our results provided evidence that <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b2 +252A\/G variant may contribute to <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content> development in a Turkish cohort. Further studies are needed to confirm this observation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22641","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T15:21:05Z","timestamp":1534951265000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of a functional variant of tumor necrosis factor\u2010\u03b2 gene in temporomandibular disorders: A pilot study"],"prefix":"10.1002","volume":"33","author":[{"given":"Kaan","family":"Yerliyurt","sequence":"first","affiliation":[{"name":"Department of Prosthetic Dentistry Faculty of Dentistry Gaziosmanpasa University  Tokat Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7639-1122","authenticated-orcid":false,"given":"Ayse Feyda","family":"Nursal","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Facultyof Medicine Hitit University  Corum 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