{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:43:29Z","timestamp":1791186209672,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,12]],"date-time":"2018-08-12T00:00:00Z","timestamp":1534032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>This study was to investigate the changes of antifungal susceptibilities caused by the phenotypic switching in patients with vulvovaginal candidiasis (<jats:styled-content style=\"fixed-case\">VVC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>229 women were enrolled in this study. The vaginal smears of these patients were collected and gram stained for fungal microscopic observation. The vaginal discharge of them in cotton swabs was cultured in sabouraud's agar with chloramphenicol medium. After fungal culture, fungal identification was analyzed using <jats:styled-content style=\"fixed-case\">CHROM<\/jats:styled-content> agar Candida chromogenic and identification medium. Then, the in vitro antifungal susceptibility testing was carried out using the standardized <jats:styled-content style=\"fixed-case\">CLSI<\/jats:styled-content> M27\u2010A2 broth microdilution method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>64.63% of <jats:italic>Candia<\/jats:italic> species in patients with <jats:styled-content style=\"fixed-case\">VVC<\/jats:styled-content> were <jats:italic>Candida albicans<\/jats:italic> and the remainders were <jats:italic>non\u2010albicans Candida<\/jats:italic> species. The phenotypic switching was observed in 91.22% of <jats:italic>C.\u00a0albicans<\/jats:italic> infection. In antifungal susceptibility testing, the susceptible rates of <jats:italic>C.\u00a0albicans<\/jats:italic> to voriconazole, fluconazole and itraconazole were significantly higher than that of <jats:italic>non\u2010albicans Candida<\/jats:italic> species (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.00, 0.00, 0.00). No matters in patients infected with <jats:italic>C.\u00a0albicans<\/jats:italic> or with <jats:italic>non\u2010albicans Candida<\/jats:italic> species<jats:italic>,<\/jats:italic> the susceptible rate to fluconazole of the clinical isolates with phenotypic switching was significantly higher than that without phenotypic switching (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01, 0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In the study, <jats:italic>C.\u00a0albicans<\/jats:italic> was the commonest pathogenic species in patients with <jats:styled-content style=\"fixed-case\">VVC<\/jats:styled-content>, in which the phenotypic switching was easy to occur. The susceptible rates of <jats:italic>C.\u00a0albicans<\/jats:italic> to all antifungal drugs were higher than that of <jats:italic>non\u2010albicans Candida<\/jats:italic> species. The susceptible rate to fluconazole was all influenced by the phenotypic switching in <jats:italic>C.\u00a0albicans<\/jats:italic> and <jats:italic>non\u2010albicans Candida<\/jats:italic> species.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22644","type":"journal-article","created":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T02:14:45Z","timestamp":1534126485000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The changes of antifungal susceptibilities caused by the phenotypic switching of <i>Candida<\/i> species in 229 patients with vulvovaginal candidiasis"],"prefix":"10.1002","volume":"33","author":[{"given":"Yuanting","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children Ministry of Education Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children Ministry of Education Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lairong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children Ministry of Education Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4740-4104","authenticated-orcid":false,"given":"Zhengqiang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children Ministry of Education Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2010.01965.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-2-1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107805"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60917-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2006.00450.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.20010"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.12994"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1135287"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2007.00549.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2010.02.027"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2011.576332"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000076"},{"key":"e_1_2_7_14_1","unstructured":"National Committee for Clinical Laboratory Standards.Reference method for broth dilution antifungal susceptibility testing of yeasts. 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