{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:14:55Z","timestamp":1783466095531,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T00:00:00Z","timestamp":1534118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The National Research Council of Thailand and Thammasat University"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Background<\/jats:title>Antibodies against human neutrophil antigens (HNA<\/jats:styled-content>s) are involved in various clinical conditions including transfusion\u2010related acute lung injury and auto\/alloimmune neutropenia. We aimed to determine HNA<\/jats:styled-content>\u20101, \u20103, \u20104, and \u20105 frequencies among southern Thais using multiplex PCR<\/jats:styled-content> and to develop HNA<\/jats:styled-content>\u20101 null detection.<\/jats:p><\/jats:sec>Methods<\/jats:title>Samples obtained from 427 southern Thai blood donors were genotyped HNA<\/jats:styled-content>\u20101, \u20103, \u20104, and \u20105 using multiplex PCR<\/jats:styled-content> and compared their allele frequencies with those previously reported in Thai populations. HNA<\/jats:styled-content>\u20101 null samples were tested by newly developed PCR<\/jats:styled-content>\u2010SSP<\/jats:styled-content> and PCR<\/jats:styled-content>\u2010RFLP<\/jats:styled-content> and confirmed by DNA<\/jats:styled-content> sequencing.<\/jats:p><\/jats:sec>Results<\/jats:title>The frequencies of FCGR<\/jats:styled-content>3B*01<\/jats:italic>, FCGR<\/jats:styled-content>3B*02<\/jats:italic>, FCGR<\/jats:styled-content>3B*03<\/jats:italic>, SLC<\/jats:styled-content>44A2*01<\/jats:italic>, SLC<\/jats:styled-content>44A2*02<\/jats:italic>, ITGAL<\/jats:styled-content>*01,<\/jats:italic> and ITGAL<\/jats:styled-content>*02<\/jats:italic> among southern Thais differed from other Thai populations, except ITGAM<\/jats:styled-content>*01<\/jats:italic> and ITGAM<\/jats:styled-content>*02<\/jats:italic> frequencies. Two samples without specific fragments of FCGR<\/jats:styled-content>3B*01<\/jats:italic>, FCGR<\/jats:styled-content>3B*02,<\/jats:italic> and FCGR<\/jats:styled-content>3B*03<\/jats:italic> tested by multiplex PCR<\/jats:styled-content> were confirmed by PCR<\/jats:styled-content>\u2010RFLP<\/jats:styled-content> to identify FCGR<\/jats:styled-content>3B<\/jats:italic> deficiency (HNA<\/jats:styled-content>\u20101 null). Moreover, to reduce test steps, the newly developed PCR<\/jats:styled-content>\u2010SSP<\/jats:styled-content> for FCGR<\/jats:styled-content>3B<\/jats:italic> deficiency was validated and tested in all samples and the results were in agreement with DNA<\/jats:styled-content> sequencing.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>This was the first study to report HNA<\/jats:styled-content>\u20101, \u20103, \u20104, and \u20105 frequencies among southern Thais. The indeterminate results of multiplex PCR<\/jats:styled-content> for HNA<\/jats:styled-content>\u20101 genotyping led to establish HNA<\/jats:styled-content>\u20101 null detection using PCR<\/jats:styled-content>\u2010SSP<\/jats:styled-content>, which is simple, convenient and cost\u2010effective and can be used to identify FCGR<\/jats:styled-content>3B<\/jats:italic> deficiency.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22651","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T02:01:25Z","timestamp":1534212085000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["HNA\u20101, \u20103, \u20104, and \u20105 genotyping using multiplex PCR<\/scp> among southern Thais: Developing continual HNA<\/scp>\u20101 null detection"],"prefix":"10.1002","volume":"33","author":[{"given":"Kamphon","family":"Intharanut","sequence":"first","affiliation":[{"name":"Graduate Program in Biomedical 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