{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T11:55:16Z","timestamp":1765886116763,"version":"3.41.2"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LY18H040001"],"award-info":[{"award-number":["LY18H040001"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cytolytic vaginosis (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>) is a common disease that results in pruritus, dyspareunia, and vulvar dysuria. However, the pathological mechanisms of the disease are still unclear. Compared to traditional methods, high\u2010throughput sequencing can obtain more accurate qualitative and quantitative information on the microbiome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected 75 samples from 32 healthy women (average age 44\u00a0\u00b1\u00a08) and 43 patients with <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> (average age 38\u00a0\u00b1\u00a08). We used high\u2010throughput sequencing of the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> V3\u2010V4 region to characterize and compare the vaginal microbiota of patients with <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> and healthy women and to identify potential biomarkers for <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The vaginal <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> of patients with <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> was \u22643.8, and the vaginal concentration of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> was \u22652\u00a0\u03bcmol\/L. Colony densities of <jats:italic>Lactobacillus<\/jats:italic> spp. in patients with <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> ranged from +++ (5\u201030) to ++++ (&gt;30) and were significantly higher than those in healthy women. High\u2010throughput sequencing showed that <jats:italic>Lactobacillus<\/jats:italic> was the most prominent genus both in patients with <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> and in healthy women, with abundances of 83.8% and 97.2%, respectively (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). <jats:italic>Lactobacillus crispatus<\/jats:italic> was more abundant in patients with <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>, whereas <jats:italic>Lactobacillus<\/jats:italic> sp. L\u2010<jats:styled-content style=\"fixed-case\">YJ<\/jats:styled-content> was more abundant in healthy women, with area under the curve (<jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>) values of 0.9375 and 0.8379, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The abundance of <jats:italic>Lactobacillus<\/jats:italic> spp. in <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> patients was significantly different from that of healthy patients. Two suitable biomarkers, <jats:italic>L<\/jats:italic>.\u00a0crispatus and <jats:italic>Lactobacillus<\/jats:italic> sp. L\u2010<jats:styled-content style=\"fixed-case\">YJ<\/jats:styled-content>, were identified. These results will be useful for the identification of women at risk of serious illness before they develop obvious symptoms.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22653","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T06:35:46Z","timestamp":1536647746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of the vaginal microbiome during cytolytic vaginosis using high\u2010throughput sequencing"],"prefix":"10.1002","volume":"33","author":[{"given":"Haihong","family":"Xu","sequence":"first","affiliation":[{"name":"Sir Run Run Shaw Hospital School of Medicine Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Women's Hospital School of Medicine Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwu","family":"Yao","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Prevention and Control  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Prevention and Control  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4070-6844","authenticated-orcid":false,"given":"Yanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Prevention and Control  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2584-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000431286"},{"issue":"9","key":"e_1_2_7_4_1","first-page":"4","article-title":"Epidemiological, clinical and microbiological findings in women with aerobic vaginitis","volume":"54","author":"Dermendjiev T","year":"2015","journal-title":"Akush Ginekol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.14209"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwx043"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01757-16"},{"issue":"3","key":"e_1_2_7_8_1","first-page":"134","article-title":"Recurrent vulvovaginal candidiasis: could it be related to cell\u2010mediated immunity defect in response to Candida antigen?","volume":"11","author":"Talaei Z","year":"2017","journal-title":"Int J Fertil Steril"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1353\/hpu.2017.0102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-017-4465-y"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173604"},{"issue":"2","key":"e_1_2_7_12_1","first-page":"113","article-title":"An epidemiological study on vaginitis in 6,150 women of reproductive age in Shanghai","volume":"40","author":"Wang H","year":"2017","journal-title":"New Microbiol"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0181477"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000446945"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7184.55490"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016349509024442"},{"issue":"1","key":"e_1_2_7_17_1","first-page":"23","article-title":"Cytolytic vaginosis: examination of 2947 vaginal smears","volume":"11","author":"Demirezen S","year":"2003","journal-title":"Cent Eur J Public Health"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10647440410001672139"},{"issue":"5","key":"e_1_2_7_19_1","first-page":"15","article-title":"Frequency of cytolytic vaginosis\u2013examination of 1152 patients","volume":"48","author":"Batashki I","year":"2009","journal-title":"Akush Ginekol"},{"issue":"2","key":"e_1_2_7_20_1","first-page":"45","article-title":"Cytolytic vaginosis and lactobacillosis. 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