{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T01:56:27Z","timestamp":1791510987841,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/00984\u20103"],"award-info":[{"award-number":["2014\/00984\u20103"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["2015\/141693\u20100"],"award-info":[{"award-number":["2015\/141693\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>There is paucity of data on the influence of alpha thalassemia on the clinical and laboratory parameters among Nigerian sickle cell anemia (<jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>) patients. This study aimed to determine the prevalence of alpha thalassemia and the influence of alpha thalassemia on laboratory parameters and clinical manifestations in a group of young Nigerian<jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a cross\u2010sectional retrospective study conducted on 100 patients with<jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>and 63 controls. The diagnosis of<jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>was confirmed by<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>studies. Alpha thalassemia genotyping was performed by multiplex gap\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>method. Laboratory parameters including complete blood count, hemoglobin quantitation, serum lactate dehydrogenase (<jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content>), and bilirubin were determined with standard techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Alpha thalassemia was found in 41 (41.0%) patients compared to 24 (38.1%) controls (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.744), and all were due to the 3.7\u00a0\u03bab \u03b1\u2010globin gene deletions. Alpha thalassemia was associated with more frequent bone pain crisis, higher hemoglobin concentration, red blood cell count, and HbA<jats:sub>2<\/jats:sub>level among the patients. On the contrary, patients with alpha thalassemia had lower mean corpuscular volume, mean corpuscular hemoglobin, and white blood cell count (<jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content>) (<jats:italic>P<\/jats:italic>\u00a0\u02c2\u00a00.05). There were 6 (6.0%) patients with leg ulcers, and none of them had alpha thalassemia,<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study confirms that coexistence of alpha thalassemia with<jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>significantly influences both the clinical and laboratory manifestations of young Nigerian<jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>patients. The coexistence of this genetic modifier is associated with increased bone pain crisis and protects against sickle leg ulcers among the patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22656","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T15:20:58Z","timestamp":1534951258000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Influence of alpha thalassemia on clinical and laboratory parameters among nigerian children with sickle cell anemia"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2564-3064","authenticated-orcid":false,"given":"Oladele S.","family":"Olatunya","sequence":"first","affiliation":[{"name":"Hematology and Hemotherapy Center University of Campinas Campinas S\u00e3o Paulo State Brazil"},{"name":"Department of Paediatrics College of Medicine Ekiti State University Ado Ekiti Ekiti State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulcineia M.","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Hematology and Hemotherapy Center University of Campinas Campinas S\u00e3o Paulo State Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adekunle","family":"Adekile","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Faculty of Medicine Kuwait University Kuwait Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando F.","family":"Costa","sequence":"additional","affiliation":[{"name":"Hematology and Hemotherapy Center University of Campinas Campinas S\u00e3o Paulo State Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61229-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/inthealth\/ihw014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2006.07.001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjhh.2014.07.018"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199609)53:1<1::AID-AJH1>3.0.CO;2-V"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000040890"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05411.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1182\/blood.V72.4.1216.1216","article-title":"Rheology predictors of the severity of the painful crisis","volume":"72","author":"Ballas SK","year":"1988","journal-title":"Blood"},{"key":"e_1_2_8_10_1","first-page":"63","article-title":"Alpha thalassemia protects sickle cell anemia patients from macro\u2010albuminuria through its effects on red blood cell rheological properties","volume":"57","author":"Lamarre Y","year":"2014","journal-title":"Clin Hemorheal Micro\u2010circ"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198206173062402"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.4.1403.1403"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21838"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.26455"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23232"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100516"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v15i2.48"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22186"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269308998887"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1987.tb00013.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017005231"},{"key":"e_1_2_8_22_1","first-page":"338","article-title":"More definitions in sickle cell disease: Steady state v baseline","volume":"86","author":"Ballas SK","year":"2011","journal-title":"Am J Hematol"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/ajh.21550","article-title":"Definitions of phenotypic manifestations of sicle cell disease","volume":"85","author":"Ballas SK","year":"2010","journal-title":"Am J Hematol"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb04639.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199808)58:4<306::AID-AJH10>3.0.CO;2-5"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199610)53:2<72::AID-AJH3>3.0.CO;2-0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572008000400003"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajhb.21047"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjhh.2014.06.002"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23811"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2001000600009"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1182\/blood.V58.5.963.963","article-title":"The diagnosis of iron deficiency anemia in sickle cell disease","volume":"58","author":"Vichinsky E","year":"1981","journal-title":"Blood"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.4084\/mjhid.2013.069"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2004.07.004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc066547"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.22065"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24134"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2010.526003"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-332205"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X2011000400004"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199111213252104"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22656","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22656","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T13:31:36Z","timestamp":1720531896000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,20]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1002\/jcla.22656"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22656","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,20]]},"assertion":[{"value":"2018-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22656"}}