{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:39:37Z","timestamp":1758638377751,"version":"3.41.2"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,27]],"date-time":"2018-08-27T00:00:00Z","timestamp":1535328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Monoclonal free light chains (<jats:styled-content style=\"fixed-case\">FLC<\/jats:styled-content>) commonly exist in monomeric or dimeric forms but rarely as larger molecules. Little is known about whether polymeric molecules can affect urine protein electrophoresis (<jats:styled-content style=\"fixed-case\">UPE<\/jats:styled-content>) results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urine samples were collected from 72 multiple myeloma (<jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content>) patients with Bence Jones protein (<jats:styled-content style=\"fixed-case\">BJP<\/jats:styled-content>). Urine protein and immunofixation electrophoresis were analyzed on Sebia <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content> \u201cagarose\u201d gel electrophoresis system (<jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content>), and immunoglobulin free light chains were measured on the <jats:styled-content style=\"fixed-case\">BNII<\/jats:styled-content> nephelometric assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A type of disulfide\u2010bound <jats:styled-content style=\"fixed-case\">FLC<\/jats:styled-content> dimer shows a pattern shift to the position of the \u201calbumin\u201d band in urine protein electrophoresis in multiple myeloma (<jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content>) patients according to the Sebia agarose gel\u2010based detection system, which was validated by immunofixation, <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>, and mass spectrometric methods. Similar cases were found in 21 (29.17%) of 72 <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> patients with <jats:styled-content style=\"fixed-case\">BJP<\/jats:styled-content>, and 19 (90.5%) of 21 patients were the lambda type.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that <jats:styled-content style=\"fixed-case\">BJP<\/jats:styled-content> with lambda type has a strong tendency to abnormally migrate, which may increase the risk of misinterpretation of protein electrophoresis in clinics. Thus, when the urine protein electrophoresis is inconsistent with the result by nephelometric method, urine protein electrophoresis needs to be repeated on the deduced condition to confirm the essence of the originally identified \u201calbumin.\u201d<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22658","type":"journal-article","created":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T21:15:03Z","timestamp":1535577303000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The <scp>FLC<\/scp> dimer with lambda type may false\u2010migrate to the position of \u201calbumin\u201d band by urine protein electrophoresis on Sebia agarose gel\u2010based detection system"],"prefix":"10.1002","volume":"33","author":[{"given":"Changqiang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Ruijin Hospital North Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peizhan","family":"Chen","sequence":"additional","affiliation":[{"name":"Translational Medicine Research Center Ruijin Hospital North Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0059-393X","authenticated-orcid":false,"given":"Xuqian","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Pathology Ruijin Hospital North Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(74)90039-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1309\/6K33KTFA7A5VUQ1T"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365517609054462"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365518009093014"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0954-6820.1984.tb05037.x"},{"issue":"10","key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1093\/clinchem\/48.10.1805","article-title":"Trimolecular complexes of lambda light chain dimers in serum of a patient with multiple myeloma","volume":"48","author":"Abraham RS","year":"2002","journal-title":"Clin 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