{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T15:46:32Z","timestamp":1777045592527,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T00:00:00Z","timestamp":1539561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81302540"],"award-info":[{"award-number":["81302540"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Heterophilic antibodies are still an important source of interference in immunoassays, but reports of interference with D\u2010dimers are rare. Are D\u2010dimer level abnormalities, found in the clinic, caused by heterophilic antibodies as well, or are other mechanisms involved? We will elaborate on this issue through two different examples in this article.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum from two patients with significantly elevated levels of D\u2010dimers were measured and compared by different methods, diluted, and dealt with heterophilic antibody blockers. At the same time, to retrieve the interference, we focused on the cause of D\u2010dimer false positives and made a systematic review of the literature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The D\u2010dimer values were normal (0.49 and 0.15\u00a0\u03bcg\/mL) detected with different testing method and decreased after addition of heterophilic antibody blocking reagent. According to literature data, there were 66.7% (4\/6) references showed the interference were heterophilic antibody.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The influence of heterophilic antibodies on the measurement of D\u2010dimers remains a big challenge. Different measuring instruments and methods may have significant differences in the measurement of D\u2010dimers. By using a combination of instrumental methods for measuring, incorporating heterophilic antibody blockers, and combining with clinical performance and imaging data, most of the interference can be eliminated.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22687","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T12:23:52Z","timestamp":1539606232000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["What makes D\u2010dimer assays suspicious\u2013heterophilic antibodies?"],"prefix":"10.1002","volume":"33","author":[{"given":"Ying","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying\u2010Xiu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Neurology the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teng\u2010Yi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian\u2010Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan\u2010Bing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7135-1391","authenticated-orcid":false,"given":"Ying\u2010E","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the First Affiliated Hospital of Shantou University Medical College Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4TB01769B"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MBC.0000000000000453"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12665"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2015.11.031"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/lmw001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2013.05.011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)01324-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/pcan.2011.58"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.09.004"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.323.7315.705"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.040501"},{"issue":"1306","key":"e_1_2_7_13_1","first-page":"130","article-title":"Heterophile antibodies and troponin results: implications in rural setting","volume":"122","author":"Narasimhan S","year":"2009","journal-title":"N Z Med J"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MBC.0000000000000017"},{"key":"e_1_2_7_15_1","first-page":"391","article-title":"When lab tests lie \u2026 heterophile antibodies","volume":"43","author":"Morton A","year":"2014","journal-title":"Aust Fam Physician"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1326782"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2012.011228"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-200109000-00012"},{"issue":"24","key":"e_1_2_7_19_1","first-page":"3439","article-title":"Investigation and analysis of false\u2010increased D\u2010dimer results: a case report","volume":"34","author":"Zhao QY","year":"2013","journal-title":"Int J Lab Med"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04515.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/ehjcvp\/pvv039"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-06-165845"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2013.08.006"},{"issue":"4","key":"e_1_2_7_24_1","first-page":"441","article-title":"Discrepancy between two methods of D\u2010dimers measurement: one case of human anti\u2010mouse antibody interference","volume":"66","author":"Rouviere JA","year":"2008","journal-title":"Ann De Bio Clin"},{"issue":"11","key":"e_1_2_7_25_1","first-page":"e253","article-title":"False\u2010positive D\u2010dimer result in a latex\u2010enhanced immunoassay caused by interfering human anti\u2010mouse antibodies","volume":"52","author":"Robier C","year":"2014","journal-title":"Clin Chem Lab Med"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22687","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22687","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T10:46:12Z","timestamp":1694688372000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22687"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,15]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1002\/jcla.22687"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22687","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,15]]},"assertion":[{"value":"2018-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22687"}}