{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:02:53Z","timestamp":1785178973766,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The DiaPlexQ\u2122 STI6 Detection Kit (DiaPlexQ; Solgent Co., Ltd., Daejeon, South Korea) is a multiplex real\u2010time PCR assay for the detection of the following sexually transmitted disease (STD) pathogens: <jats:italic>Chlamydia trachomatis<\/jats:italic>, <jats:italic>Neisseria gonorrhoeae<\/jats:italic>, <jats:italic>Mycoplasma hominis<\/jats:italic>, <jats:italic>Trichomonas vaginalis<\/jats:italic>, <jats:italic>Ureaplasma urealyticum<\/jats:italic>, and <jats:italic>Mycoplasma genitalium<\/jats:italic>. We compared the performance of the DiaPlexQ assay with the GeneFinder\u2122 STD I (CT\/NG\/UU) and STD II (MG\/MH\/TV) Multiplex Real\u2010time PCR Kits (GeneFinder; Infopia Co., Ltd., Anyang, South Korea).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated the performance of the DiaPlexQ assay in comparison to that of GeneFinder using 1106 clinical specimens (542 genital swabs and 564 urine samples). The analytical performance of the DiaPlexQ assay, including the limit of detection (LOD) and analytical specificity, was evaluated using reference strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The positive percent agreement, negative percent agreement, and kappa value between the two assays were 96.6%\u201099.4%, 98.2%\u201099.8%, and 0.93%\u20100.99%, respectively. No cross\u2010reactivity was observed in a collection of 41 different microorganisms and the LOD of the DiaPlexQ assay ranged from 1 to 10 copies\/reaction for each microorganism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The DiaPlexQ assay showed comparable performance to that of the GeneFinder assay so that it can be used for the screening and diagnosis of non\u2010viral curable STD pathogens.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22703","type":"journal-article","created":{"date-parts":[[2018,11,3]],"date-time":"2018-11-03T06:54:47Z","timestamp":1541228087000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison between DiaPlexQ\u2122 STI6 and GeneFinder\u2122 STD I\/STD II multiplex Real\u2010time PCR Kits in the detection of six sexually transmitted disease pathogens"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8999-7561","authenticated-orcid":false,"given":"Hee Jae","family":"Huh","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Genetics, Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Seok","family":"Ki","sequence":"additional","affiliation":[{"name":"Green Cross Genome Yongin South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Ae","family":"Yun","sequence":"additional","affiliation":[{"name":"Center for Clinical Medicine, Samsung Biomedical Research Institute Samsung Medical Center Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungsoo","family":"Lee","sequence":"additional","affiliation":[{"name":"Eone Laboratories Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwi Young","family":"Oh","sequence":"additional","affiliation":[{"name":"Eone Laboratories Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam\u2010Sihk","family":"Lee","sequence":"additional","affiliation":[{"name":"Eone Laboratories Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Ho","family":"Yoon","sequence":"additional","affiliation":[{"name":"Eone Laboratories Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics, Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,2]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"World Health Organization.Global health sector strategy on sexually transmitted infections 2016\u20132021.https:\/\/www.who.int\/reproductivehealth\/publications\/rtis\/ghss-stis\/en\/(Updated on July 2016). Accessed September 24 2018."},{"key":"e_1_2_5_3_1","unstructured":"Centers for Disease Control and Prevention.Sexually transmitted diseases treatment guidelines 2015.https:\/\/www.cdc.gov\/std\/tg2015\/tg-2015-print.pdf(Update on June 2015). Accessed September 24 2018."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30310-9"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819071"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01766-16"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.06.005"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2014.11.010"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12430"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2646-4"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01430-17"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2013.07.011"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.871495"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.5145\/ACM.2017.20.1.1"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01962-15"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/clinchem\/45.6.777","article-title":"Strand displacement amplification and homogeneous real\u2010time detection incorporated in a second\u2010generation DNA probe system. BDProbeTecET","volume":"45","author":"Little MC","year":"1999","journal-title":"Clin Chem"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03552-13"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00519-13"},{"key":"e_1_2_5_19_1","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01091-17","article-title":"Clinical evaluation of the Cepheid Xpert TV Assay for detection of Trichomonas vaginalis with prospectively collected specimens from men and women","volume":"56","author":"Schwebke JR","year":"2018","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03461-12"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22703","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22703","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.22703","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T07:34:58Z","timestamp":1694417698000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,2]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/jcla.22703"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22703","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,2]]},"assertion":[{"value":"2018-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22703"}}