{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T13:21:13Z","timestamp":1770297673505,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T00:00:00Z","timestamp":1541462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["registration#161749"],"award-info":[{"award-number":["registration#161749"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>CD40 is a costimulatory molecule for B cells, and CD154 is a marker of CD4+ T cells activation. CD40\u2010CD154 interaction promotes pro\u2010inflammatory cytokines secretion and autoantibodies production. <jats:italic>PTPN22<\/jats:italic> gene encodes LYP protein, an inhibitor of T\u2010 and B\u2010cell activation. <jats:italic>PTPN22<\/jats:italic> 1858C&gt;T polymorphism confers rheumatoid arthritis (RA) susceptibility. Hence, we evaluate the relationship between 1858C&gt;T polymorphism with CD40 and CD154 expression and IFN\u2010\u03b3 secretion in RA patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>PTPN22<\/jats:italic> 1858C&gt;T polymorphism was genotyped in 315 RA patients and 315 control subjects (CS) using PCR\u2010RFLP method. Later, we selected only ten anti\u2010CCP\u2010positive RA patients, na\u00efve to disease\u2010modifying antirheumatic drugs and ten CS, all with known 1858C&gt;T <jats:italic>PTPN22<\/jats:italic> genotype. The CD40 and CD154 membrane expressions were determined by flow cytometry in peripheral B and T cells, correspondingly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The B cells percentage and mCD40 expression were similar between RA and CS (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05) and we did not find an association between these variables and the 1858C&gt;T polymorphism. The CD4+ T cells percentage was higher in RA patients than CS (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003), and in the RA group, the CD4+ T cells percentage and mCD154 expression were higher in the 1858\u00a0T allele carriers (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.008 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.032, respectively). The IFN\u2010\u03b3 levels were lower in RA patients carrying the <jats:italic>PTPN22<\/jats:italic> risk allele (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.032).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:italic>PTPN22<\/jats:italic> 1858\u00a0T risk allele is associated with increased CD4+ T cells percentage and high mCD154 expression in RA patients, which could favor the pro\u2010inflammatory cytokine release and the establishment of the inflammatory response at the seropositive RA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22710","type":"journal-article","created":{"date-parts":[[2018,11,7]],"date-time":"2018-11-07T06:24:55Z","timestamp":1541571895000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>PTPN22<\/i> 1858C&gt;T polymorphism is associated with increased CD154 expression and higher CD4+ T cells percentage in rheumatoid arthritis patients"],"prefix":"10.1002","volume":"33","author":[{"given":"Yeniley","family":"Ruiz\u2010Noa","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Ciencias Biom\u00e9dicas CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8004-1811","authenticated-orcid":false,"given":"Jorge","family":"Hern\u00e1ndez\u2010Bello","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Ciencias Biom\u00e9dicas CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6059-673X","authenticated-orcid":false,"given":"Mara A.","family":"Llamas\u2010Covarrubias","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Ciencias Biom\u00e9dicas CUCS, Universidad de Guadalajara Guadalajara 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