{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:27Z","timestamp":1786977987631,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,25]],"date-time":"2019-02-25T00:00:00Z","timestamp":1551052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and technology Bureau of Zhejiang Province","award":["LGD19H080002"],"award-info":[{"award-number":["LGD19H080002"]}]},{"name":"Education Bureau of Zhejiang Province","award":["Y201636005"],"award-info":[{"award-number":["Y201636005"]}]},{"name":"Health Bureau of Zhejiang Province","award":["2016KYB154"],"award-info":[{"award-number":["2016KYB154"]}]},{"name":"Health Bureau of Zhejiang Province","award":["2017KY419"],"award-info":[{"award-number":["2017KY419"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The colonization of\n                      <jats:italic>Ureaplasma<\/jats:italic>\n                      species in genital tract is related with male infertility. However, it has been postulated based upon limited study that virulence is related to serotype specificity. The aim of this study was to determine the distribution of\n                      <jats:italic>Ureaplasma<\/jats:italic>\n                      serovars in genital tract of infertile males and analyze their role in male infertility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 358 urethral swabs samples were obtained from infertile males. The culture of\n                      <jats:italic>Ureaplasma<\/jats:italic>\n                      species were performed using a commercially available Mycoplasma IST 2 kit. Serovars were determined by real\u2010time polymerase chain reaction (real\u2010time PCR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 92 (25.7%) infertile males were positive for\n                      <jats:italic>Ureaplasma<\/jats:italic>\n                      spp; among them,\n                      <jats:italic>Ureaplasma parvum<\/jats:italic>\n                      (UPA) was detected in 73 (79.3%) isolates, and\n                      <jats:italic>Ureaplasma urealyticum<\/jats:italic>\n                      (UUR) was detected in 19 (20.7%) isolates. Serovars 1, 6, or in combination accounted for 63.0% (46\/73) of UPA isolates. Serovar 9 (alone and in combination of other serovars) was the most common serovar in UUR (47.4%, 9\/19). Multiple serovars were detected in 21 (22.8%) isolates, and serovars 4, 5, 7, and 12 were not detected in any sample.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The distribution of 14\n                      <jats:italic>Ureaplasma<\/jats:italic>\n                      serovars in genital tract of infertile males was identified for the first time by real\u2010time PCR assay. UPA serovars 1 and 6, and UUR serovar 9 are the most common serovars colonization in urogenital tract of infertile males.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.22865","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T01:27:05Z","timestamp":1551144425000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of\n                    <i>Ureaplasma<\/i>\n                    spp. serovars in genital tract of infertile males"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2254-591X","authenticated-orcid":false,"given":"Tiejun","family":"Song","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Sir Run Run Shaw hospital, School of medicine Zhejiang University Hangzhou 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