{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:42:19Z","timestamp":1791654139600,"version":"4.3.4"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T00:00:00Z","timestamp":1553817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572067"],"award-info":[{"award-number":["81572067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601834"],"award-info":[{"award-number":["81601834"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702073"],"award-info":[{"award-number":["81702073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Innate immunity plays a crucial role in host\u2010virus interactions and greatly influences viral replication including HBV infection. However, few studies have investigated the possible antiviral immune roles played by TLRs, RIG\u2010I, and long no\u2010coding RNA NEAT1 in chronic HBV infection (CHB) patients in clinical samples and their relationships among immune responses. In this study, we sought to investigate the mRNA expression levels of TLR1\u201010, RIG\u2010I, and NEAT1 expression in HBeAg\u2010positive CHB treatment\u2010na\u00efve patients with the active phase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression levels of TLR1\u201010, RIG\u2010I, and NEAT1 of CHB patients with the active phase and healthy controls were measured by qPCR. Serum HBV DNA and routine liver biochemistry including ALT, etc were also measured to evaluate the impaired physiological function of the liver affected by CHB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression levels of TLR1 and TLR6 in CHB with active phase were remarkably lower than that in healthy controls. The levels of TLR3 in CHB patients with active phase were remarkably higher than that in healthy controls. The total NEAT1 expression was abnormally decreased in CHB patients as compared with healthy controls. The levels of RIG\u2010I were significantly decreased in CHB patients in the active phase when compared to healthy controls. The expression of TLR6 and RIG\u2010I was closely correlated with NEAT1 expression. TLR6 level was positively correlated with RIG\u2010I level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Chronic HBV infection can alter the innate immune response by downregulating functional expression of TLR1, TLR6, NEAT1.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.22886","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T12:46:59Z","timestamp":1553863619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Toll\u2010like receptors, long non\u2010coding RNA NEAT1, and RIG\u2010I expression are associated with HBeAg\u2010positive chronic hepatitis B patients in the active phase"],"prefix":"10.1002","volume":"33","author":[{"given":"Yongbin","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Department of Gene Diagnosis Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wennan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Department of Gene Diagnosis Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Department of Gene Diagnosis Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanjian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Department of Gene Diagnosis Fujian Medical University Fuzhou 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