{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:06:56Z","timestamp":1790172416883,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T00:00:00Z","timestamp":1563235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T00:00:00Z","timestamp":1563235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Key Research Laboratory Support Project of Hebei Construction Commission","award":["Hebei traditional Chinese Medicine [2014] 28 -14"],"award-info":[{"award-number":["Hebei traditional Chinese Medicine [2014] 28 -14"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In China, the incidence of autoimmune diseases is gradually increasing. To decrease the misdiagnosis rate of autoimmune diseases, we conducted an epidemiological investigation about the presence of antinuclear antibody (ANA) in healthy populations and analyzed the clinical characteristics of healthy population with both high titer of ANA and positive anti\u2010SSA and AMA\u2010M2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum ANA titers were detected by indirect immunofluorescence (IIF), and other 15 types of ANA\u2010specific antibodies were detected by line immunoassays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In 25\u00a0110 individuals for routine examination, the positive rate of ANA titer &gt;1:100 was 14.01%, of which the positive rate of female (19.05%) was higher than that of male (9.04%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). The positive rate of ANA titer &gt;1:320 was 5.93%, of which the positive rate of female (8.68%) was higher than that of male (3.21%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). The specific antibodies were detected in 1489 of ANA\u2010positive people with titer &gt;1:320, and the top three detected antibodies were anti\u2010Ro\u201052 (212), AMA\u2010M2 (189), and anti\u2010SSA (144). The abnormal rate of blood routine test, liver function test, and other clinical indicators in AMA\u2010M2\u2013positive population was significantly different from those in the control group. The abnormal rate of blood routine test, liver function test, and immune index in anti\u2010SSA\u2013positive population was higher than those in control group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>There was a high prevalence of ANA positive in healthy population. To avoid misdiagnosis, those who had symptoms of abdominal discomfort, pruritus, or fatigue with abnormal results of blood routine and liver function test should be examined for ANA, AMA\u2010M2, anti\u2010SSA as early as possible.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.22965","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T02:14:21Z","timestamp":1563329661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Epidemiological survey of antinuclear antibodies in healthy population and analysis of clinical characteristics of positive population"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5851-2912","authenticated-orcid":false,"given":"Xiaoyan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Baoding NO.1 Central Hospital Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Baoding NO.1 Central Hospital Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Baoding NO.1 Central Hospital Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Baoding NO.1 Central Hospital Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liang","sequence":"additional","affiliation":[{"name":"Chengde Medical University Chengde China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Baoding NO.1 Central Hospital Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2614-3048","authenticated-orcid":false,"given":"Yaping","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Baoding NO.1 Central Hospital Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2001.00959.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)04410-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i29.3616"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34380"},{"issue":"11","key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.4049\/jimmunol.139.11.3828","article-title":"Molecular cloning of the liver\u2010specific rat F antigen","volume":"139","author":"Gershwin ME","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21583"},{"issue":"6","key":"e_1_2_6_8_1","first-page":"1037","article-title":"Quantitative fibrosis parameters highly predict esophageal\u2010gastro varices in primary biliary cirrhosis","volume":"20","author":"Wu QM","year":"2016","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22906"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.04.009"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00154-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780331111"},{"issue":"3","key":"e_1_2_6_13_1","first-page":"165","article-title":"The clinical significance of SSA antigen and its different positive expressions","volume":"42","author":"Li YJ","year":"2003","journal-title":"Zhonghua Nei Ke Za Zhi"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01195.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/hs0402.105927"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.22965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.22965","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.22965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:40:37Z","timestamp":1790170837000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.22965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,16]]},"references-count":14,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/jcla.22965"],"URL":"https:\/\/doi.org\/10.1002\/jcla.22965","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,16]]},"assertion":[{"value":"2019-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22965"}}