{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:51:54Z","timestamp":1792461114214,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T00:00:00Z","timestamp":1573603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The various virulence factors of methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> bacteremia (MRSAB) are associated with a high mortality rate worldwide. Further studies are warranted to confirm the significant relationship between the strains and virulence genes. Here, we prospectively investigated the molecular characteristics underlying the genotypes and virulence factors of MRSA isolated from patients with bacteremia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected 59 MRSA isolates from adult patients with bacteremia. Antimicrobial susceptibility results were obtained with the Vitek2 automated system. Genotypes were identified with multi\u2010locus sequence typing (MLST) and pulse\u2010field gel electrophoresis (PFGE), and 21 virulence genes were detected with polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The 59 MRSA isolates mainly comprised ST5 (n\u00a0=\u00a031, 52.5%) and ST72 (n\u00a0=\u00a022, 37.2%). Most ST5 isolates and all ST72 isolates were clustered into one and two PFGE groups, respectively. The mean number of virulence genes was higher in ST5 than in ST72. <jats:italic>Sel<\/jats:italic> was more frequently detected in ST5 than in ST72, whereas <jats:italic>sec<\/jats:italic> and <jats:italic>sed<\/jats:italic> were found only in ST5. ST5 had significantly higher resistance against many antibiotics than ST72.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Most MRSA isolates causing bacteremia were ST5 (CC5) and ST72 (CC8), and those belonging to the same STs were divided into only a few PFGE groups. ST5 was associated with higher antibiotic resistance and staphylococcal superantigen toxin genes, than ST72, which may be related to its higher virulence.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23077","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T07:40:43Z","timestamp":1573630843000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Molecular epidemiology and virulence factors of methicillin\u2010resistant <i>Staphylococcus aureus<\/i> isolated from patients with bacteremia"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1725-2573","authenticated-orcid":false,"given":"Hyun Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Chungnam National University Hospital Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8520-3119","authenticated-orcid":false,"given":"Qute","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chungnam National University Hospital Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gye Cheol","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chungnam National University Hospital Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Hoe","family":"Koo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chungnam National University Hospital Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,13]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"132","article-title":"Methicillin\u2010resistant Staphylococcus aureus (MRSA) bacteremia: Correlations between clinical, phenotypic, genotypic characteristics and mortality in a tertiary teaching hospital in Malaysia","volume":"59","author":"Sit PS","year":"2018","journal-title":"Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-5-2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12705"},{"key":"e_1_2_5_5_1","unstructured":"Korea Centers for Disease Control&Prevention (KCDC).Korean antimicrobial resistance monitoring system 2016 annual report.2018."},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00221"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0147-4"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2016.351"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0b013e31822f0b54"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq373"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/345476"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2001.tb143562.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.162.19.2229"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2010.07.011"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b16-00255"},{"issue":"4","key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1093\/jac\/dku475","article-title":"Community\u2010associated MRSA strain ST72\u2010SCCmecIV causing bloodstream infections: clinical outcomes and bacterial virulence factors","volume":"70","author":"Park K\u2010H","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.3343\/kjlm.2011.31.1.30"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0468"},{"key":"e_1_2_5_19_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. 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