{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:37:31Z","timestamp":1778931451285,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T00:00:00Z","timestamp":1572220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recurrent spontaneous abortion (RSA) is a serious problem in pregnancy. The exact etiology of RSA is unknown in more than 50% of all the patients. However, genetic variations are known as susceptibility factors for idiopathic RSA. Considering the role of miRNA biosynthesis machinery in the miRNA production and effect of miRNAs on various diseases, this study aimed to evaluate the effects of <jats:italic>DICER1<\/jats:italic> rs3742330 and <jats:italic>DROSHA<\/jats:italic> rs6877842 polymorphisms on RSA risk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this case\u2010control study, 150 RSA patients and 195 age\u2010matched healthy female controls were recruited. Both polymorphisms were genotyped using PCR\u2010RFLP method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of <jats:italic>DICER1<\/jats:italic> rs3742330AG genotype was higher in the control group (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.022). There was a statistically significant association between rs3742330 polymorphism and a reduced RSA risk in dominant and allelic models (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.013 and <jats:italic>P<\/jats:italic>\u00a0=\u00a0.007, respectively). No statistically significant association was found between <jats:italic>DROSHA<\/jats:italic> rs6877842 variant and RSA risk. The combination of AG and GC genotypes and G\u2010G alleles of <jats:italic>DICER1<\/jats:italic> rs3742330 and <jats:italic>DROSHA<\/jats:italic> rs6877842 polymorphisms led to a decreased RSA risk. However, the synergic effect of rs3742330A and rs6877842G alleles (A\u2010G) and AA\u2010GG genotypes was associated with an increased RSA risk.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>the <jats:italic>DICER1<\/jats:italic> rs3742330AG genotype and combination of AG and GC genotypes and G\u2010G alleles of <jats:italic>DICER1<\/jats:italic> rs3742330 and <jats:italic>DROSHA<\/jats:italic> rs6877842 polymorphisms were associated with a reduced RSA risk.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23079","type":"journal-article","created":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T01:01:52Z","timestamp":1572310912000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The effects of <i>DICER1<\/i> and <i>DROSHA<\/i> polymorphisms on susceptibility to recurrent spontaneous abortion"],"prefix":"10.1002","volume":"34","author":[{"given":"Marzieh","family":"Ghasemi","sequence":"first","affiliation":[{"name":"Pregnancy Health Research Center Zahedan University of Medical Sciences Zahedan Iran"},{"name":"Moloud Infertility Center Ali ibn Abitaleb Hospital Zahedan University of Medical Sciences Zahedan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahnaz","family":"Rezaei","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry School of Medicine Zahedan University of Medical Sciences Zahedan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atefeh","family":"Yazdi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology School of Medicine Zahedan University of Medical Sciences Zahedan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narjes","family":"Keikha","sequence":"additional","affiliation":[{"name":"Moloud Infertility Center Ali 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